Scaled-Up Inertial Microfluidics: Retention System for Microcarrier-Based Suspension Cultures

Biotechnology Journal
Reza MoloudiMay Win Naing

Abstract

Recently, particle concentration and filtration using inertial microfluidics have drawn attention as an alternative to membrane and centrifugal technologies for industrial applications, where the target particle size varies between 1 µm and 500 µm. Inevitably, the bigger particle size (>50 µm) mandates scaling up the channel cross-section or hydraulic diameter (DH > 0.5 mm). The Dean-coupled inertial focusing dynamics in spiral microchannels is studied broadly; however, the impacts of secondary flow on particle migration in a scaled-up spiral channel is not fully elucidated. The mechanism of particle focusing inside scaled-up rectangular and trapezoidal spiral channels (i.e., 5-10× bigger than conventional microchannels) with an aim to develop a continuous and clog-free microfiltration system for bioprocessing is studied in detail. Herein, a unique focusing based on inflection point without the aid of sheath flow is reported. This new focusing mechanism, observed in the scaled-up channels, out-performs the conventional focusing scenarios in the previously reported trapezoidal and rectangular channels. Finally, as a proof-of-concept, the utility of this device is showcased for the first time as a retention system for a cell-micr...Continue Reading

References

Nov 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Dino Di CarloMehmet Toner
Oct 21, 2009·Journal of Tissue Engineering and Regenerative Medicine·D SchopJ D de Bruijn
Jan 22, 2010·Lab on a Chip·Soojung Claire HurDino Di Carlo
Jul 1, 2010·Biotechnology and Bioengineering·Albert J Mach, Dino Di Carlo
Jul 19, 2011·Stem Cell Research·Allen Kuan-Liang ChenSteve Kah Weng Oh
Jul 20, 2011·Biotechnology Letters·Christopher J HewittColin R Thomas
Mar 3, 2012·Journal of Tissue Engineering and Regenerative Medicine·Sébastien SartSpiros N Agathos
Mar 29, 2012·Physics of Fluids·Joseph M Martel, Mehmet Toner
Apr 24, 2013·Biotechnology Letters·Qasim A RafiqChristopher J Hewitt
Aug 21, 2013·Lab on a Chip·Majid Ebrahimi WarkianiJongyoon Han
Jan 10, 2014·Biomicrofluidics·Nivedita Nivedita, Ian Papautsky
Jun 4, 2014·Biotechnology and Bioengineering·Julia HupfeldHeike Wegmeyer
Jun 7, 2014·Annual Review of Biomedical Engineering·Joseph M Martel, Mehmet Toner
Jun 11, 2014·Lab on a Chip·Hamed AminiDino Di Carlo
Jun 11, 2015·Scientific Reports·Joseph M MartelMehmet Toner
Jul 15, 2015·Scientific Reports·Majid Ebrahimi WarkianiJongyoon Han
Jan 23, 2016·Lab on a Chip·Chao LiuGuoqing Hu
Jul 6, 2016·Lab on a Chip·Mehdi RafeieMajid Ebrahimi Warkiani
Dec 20, 2016·Scientific Reports·Arzu ÖzbeyAli Koşar
Jul 29, 2017·Scientific Reports·Taehong KwonJongyoon Han
Jun 22, 2018·Scientific Reports·Jian ZhouXuesong Ye
Jul 13, 2018·Analytical Chemistry·Nan XiangZhonghua Ni

❮ Previous
Next ❯

Citations

Nov 9, 2019·Biomicrofluidics·N HerrmannM Birkholz
Jul 17, 2020·Frontiers in Bioengineering and Biotechnology·Ang-Chen TsaiYan Li
Apr 5, 2020·Scientific Reports·Sajad Razavi BazazMajid Ebrahimi Warkiani

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.