Scaling down: a PCR-based method to efficiently screen for desired knockouts in a high density Mycobacterium tuberculosis picked mutant library

Tuberculosis
James M Lane, Eric J Rubin

Abstract

Transposon mutagenesis produces random mycobacterial mutants at high frequency. Because they are random, however, it is difficult to isolate mutations in particular target genes. Here we describe the use of an arrayed library of Mycobacterium tuberculosis together with a PCR screening strategy to rapidly identify strains with defined insertion mutations. This method is useful for many genetic applications.

References

Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·V PelicicC Guilhot
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·S BardarovW R Jacobs
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·D J LampeH M Robertson
Sep 6, 2000·Letters in Applied Microbiology·G HuangR G Birch
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·C M SassettiE J Rubin
May 31, 2003·Proceedings of the National Academy of Sciences of the United States of America·Gyanu LamichhaneWilliam R Bishai
Jun 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jyothi RengarajanEric J Rubin

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