Scanning and transmission electron microscopy of the cells forming the hepatic sinusoidal wall of rat in acetaminophen and Escherichia coli endotoxin-induced hepatotoxicity

Journal of Microscopy and Ultrastructure
Allam Mohamed NafadyHemmat Haridy Ghafeer

Abstract

Drugs and xenobiotics as well as bacterial endotoxins may reach the liver either systematically or after intestinal absorption. Therefore, cells lining the sinusoidal wall form the last barrier before blood constituents get in contact with the parenchymal cells. In this work, the ultrastructure of the cells forming the sinusoidal wall was studied after acetaminophen and Escherichia coli endotoxin treatments. Rats received acetaminophen at a dose of 1000 mg/kg body weight by intraperitoneal injection once in acute and four times with a 1-week interval in chronic treatments, and E. coli endotoxin at a dose of 5 mg/kg of body weight by intraperitoneal injection once in acute and four times with a 1-week interval in chronic treatments. Tissue samples were collected for scanning and transmission electron microscopy. Swelling of sinusoidal endothelial cells was noticed in both acute intoxicated groups with narrowing of the fenestrae, whereas large gaps were formed in chronic toxicity. Activation of Kupffer cells was a prominent common feature between the four toxicity groups. Interestingly, hepatic stellate cell activation was evident in both chronic acetaminophen and chronic endotoxin groups. Large amounts of collagen fibers were se...Continue Reading

References

Jan 1, 1996·Toxicologic Pathology·E WisseA Vermoesen
Nov 20, 2002·Comparative Hepatology·Filip Braet, Eddie Wisse
Apr 30, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Paola BrunDiego Martines
May 20, 2008·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Robert S McCuskey
Jul 30, 2008·Toxicology·Scott L Friedman
Dec 17, 2008·Biochimica Et Biophysica Acta·William S BlanerJenny Libien
Jan 2, 2009·International Urology and Nephrology·Yusuf Ozlem IlbeyFatih Mete
Jan 13, 2009·International Journal of Experimental Pathology·Rajkumar CheluvappaSarah N Hilmer
Feb 10, 2010·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Alessandra WarrenDavid G Le Couteur
Jul 9, 2010·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Rajkumar CheluvappaDavid G Le Couteur
Apr 27, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Ningling KangVijay H Shah
Aug 14, 2012·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Bilge DepboyluMüjdat Uysal
Jul 25, 2014·Journal of Molecular Endocrinology·Qi ZhangRuilan Niu
Apr 11, 2015·Toxicology Letters·Hartmut Jaeschke, Mitchell R McGill
Jan 15, 2016·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Steven A SeifertKennon J Heard

❮ Previous
Next ❯

Citations

Oct 28, 2016·Ultrastructural Pathology·Omar Bauomy AhmedSalah Mohamed Hassan Afifi

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy
scanning electron microscopy

Related Concepts

Related Feeds

Addiction

This feed focuses mechanisms underlying addiction and addictive behaviour including heroin and opium dependence, alcohol intoxication, gambling, and tobacco addiction.