Scanning Electron Microscopy Sample Preparation and Imaging

Methods in Molecular Biology
Jenny Ngoc Tran Nguyen, Amanda M Harbison

Abstract

Scanning electron microscopes allow us to reach magnifications of 20-130,000× and resolve compositional and topographical images with intense detail. These images are created by bombarding a sample with electrons in a focused manner to generate a black and white image from the electrons that bounce off of the sample. The electrons are detected using positively charged detectors. Scanning electron microscopy permits three-dimensional imaging of desiccated specimens or wet cells and tissues by using variable pressure chambers. SEM ultrastructural analysis and intracellular imaging supplement light microscopy for molecular profiling of prokaryotes, plants, and mammals. This chapter demonstrates how to prepare and image samples that are (a) desiccated and conductive, (b) desiccated and nonconductive but coated with an electron conductive film using a gold sputter coater, and

Citations

Jul 28, 2020·Frontiers in Neurology·Stephen J BlackbandJohn R Forder
Aug 28, 2020·The American Journal of Forensic Medicine and Pathology·Guendalina GentileRiccardo Zoja
Mar 2, 2021·Journal of Forensic and Legal Medicine·Debora MazzarelliCristina Cattaneo
May 12, 2021·Scientific Reports·Ahmed Fathy YousefFaxing Chen

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