PMID: 7518486Jun 24, 1994Paper

Scanning for T helper epitopes with human PBMC using pools of short synthetic peptides

Journal of Immunological Methods
J C ReeceS J Rodda

Abstract

Major T helper epitopes of medically important antigens can be located by measuring the proliferative responses of human peripheral blood mononuclear cells (PBMC) to pools of short synthetic peptides. The length and endings of the peptides used were shown to be critical for success in identifying Th cell epitopes. Many epitopes would be missed if either long (31mers) or short (less than 12mers) peptides were used. Pools of 14 and 16mers were more efficient than 12mers spanning the same region, however, for a promiscuous Th cell epitope of tetanus toxin (tt 947-967), two of three donors tested did not respond to 18mers or shorter peptides spanning this region. Although peptides with either unblocked or blocked ends were stimulatory, peptides with blocked ends were generally more efficient. The peptide concentration and number of available APC were also found affect the efficiency of the proliferation assay as a measure of peptide recognition by Th cells. Two screenings of the entire set of tetanus toxin peptide pools using different samples of PBMC from the same donor identified common major stimulatory regions. Thus, PBMC and peptide pools can be used for the reproducible identification of Th cell epitopes. After immunization w...Continue Reading

References

Jul 1, 1978·European Journal of Immunology·M H van OersW P Zeijlemaker
Jan 1, 1992·European Journal of Immunology·S R BurrowsD J Moss
Mar 1, 1990·European Journal of Immunology·P C HoC M Rzepczyk
Nov 6, 1990·Journal of Immunological Methods·N J MaejiH M Geysen
Nov 1, 1991·European Journal of Immunology·T Y NakagawaR A Flavell
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M F GoodL H Miller
Aug 15, 1989·Journal of Immunological Methods·S DemotzG Corradin
Jan 1, 1989·Cold Spring Harbor Symposia on Quantitative Biology·P Panina-BordignonA Lanzavecchia
Jun 1, 1984·Human Immunology·E J Hensen, D Elferink

❮ Previous
Next ❯

Citations

Jul 24, 2002·Journal of Immunological Methods·Stuart J Rodda
Jul 24, 2002·Journal of Immunological Methods·Eva BorràsClemencia Pinilla
Nov 13, 1996·Journal of Immunological Methods·K BromanM Tigges
Jan 11, 2003·Clinical and Diagnostic Laboratory Immunology·Liliane GoetschNathalie Corvaia
Jul 10, 2010·Journal of Biomedicine & Biotechnology·Giuseppina Li PiraFabrizio Manca
Feb 19, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·W D MüllerL Jäger
Jan 3, 2013·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M PascalH A Sampson
May 20, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·F Harding
Mar 1, 2002·BMC Immunology·Jeanette M MuchaFiona A Harding
Apr 25, 2008·Current Protocols in Immunology·S J Rodda
Jun 1, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Mary L DisisKathy Schiffman
Apr 23, 2008·Current Protocols in Protein Science·S J Rodda

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.