Scanning the landscape of genome architecture of non-O1 and non-O139 Vibrio cholerae by whole genome mapping reveals extensive population genetic diversity

PloS One
Carol ChapmanShanmuga Sozhamannan

Abstract

Historically, cholera outbreaks have been linked to V. cholerae O1 serogroup strains or its derivatives of the O37 and O139 serogroups. A genomic study on the 2010 Haiti cholera outbreak strains highlighted the putative role of non O1/non-O139 V. cholerae in causing cholera and the lack of genomic sequences of such strains from around the world. Here we address these gaps by scanning a global collection of V. cholerae strains as a first step towards understanding the population genetic diversity and epidemic potential of non O1/non-O139 strains. Whole Genome Mapping (Optical Mapping) based bar coding produces a high resolution, ordered restriction map, depicting a complete view of the unique chromosomal architecture of an organism. To assess the genomic diversity of non-O1/non-O139 V. cholerae, we applied a Whole Genome Mapping strategy on a well-defined and geographically and temporally diverse strain collection, the Sakazaki serogroup type strains. Whole Genome Map data on 91 of the 206 serogroup type strains support the hypothesis that V. cholerae has an unprecedented genetic and genomic structural diversity. Interestingly, we discovered chromosomal fusions in two unusual strains that possess a single chromosome instead of t...Continue Reading

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Citations

Oct 27, 2015·Frontiers in Microbiology·Md Habibur RahamanMunirul Alam
Apr 12, 2019·Scientific Reports·Matthew J DormanNicholas R Thomson
Dec 19, 2018·Frontiers in Microbiology·Matthias BruhnTorsten Waldminghaus
Nov 27, 2018·Frontiers in Microbiology·Matthew J Dorman, Charles J Dorman
Jul 6, 2019·Frontiers in Microbiology·Bhabatosh Das, Dhruba K Chattoraj
Apr 3, 2020·Environmental Microbiology·Shanmuga Sozhamannan, Torsten Waldminghaus
Aug 12, 2020·Journal of Bacteriology·Dhrubajyoti NagJeffrey H Withey

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Datasets Mentioned

BETA
CP001233.1
CP001234.1

Methods Mentioned

BETA
Electrophoresis

Software Mentioned

STRUCTURE
MapSolver
OpGen MapSolver
R package
ARGUS Map Solver

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