PMID: 2120834Aug 1, 1990Paper

Scanning tunneling microscopy of the enzymes of muscle glycogenolysis

Ultramicroscopy
R D EdstromD F Evans

Abstract

Scanning tunneling microscopy (STM) has been used to examine the structures of the skeletal muscle enzymes phosphorylase and phosphorylase kinase. The interaction of these two proteins represents the last step in the process of signal transduction which results in muscle glycogen being converted into metabolic energy for use in muscle contraction. Phosphorylase b has a molecular weight of 97,000 and the dimer is seen by STM to have dimensions of 11 X 5.7 nm. Phosphorylase b has a tendency to form linear arrays of dimers on the graphite surface used as the support for STM imaging. Phosphorylase kinase is imaged as a butterfly-like object with lateral dimensions of 36 X 27 nm. The molecular thicknesses given by scanning tunneling microscopy for these two non-conducting molecules is significantly less than expected. The height measurement in STM is dependent not only on the surface topology of the object being imaged, but also on the electronic work function of the object compared to that of the graphite surface on which it lies. In addition to the individual proteins, a complex between phosphorylase and phosphorylase kinase has been observed by scanning tunneling microscopy.

References

Apr 28, 1989·Science·G LeeD F Evans
Mar 31, 1989·Science·M AmreinG Travaglini
Feb 20, 1985·Journal of Molecular Biology·H J Schramm, H P Jennissen
Mar 1, 1968·Biochemistry·D A ChignellR C Valentine

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Citations

Jan 1, 1994·Progress in Biophysics and Molecular Biology·V J Morris

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