PMID: 7517921Jul 15, 1994Paper

Scattered micrometastases visualized at the single-cell level: detection and re-isolation of lacZ-labeled metastasized lymphoma cells

International Journal of Cancer. Journal International Du Cancer
Achim KrügerP Von Hoegen

Abstract

To study metastasis at the single-cell level we transduced highly metastatic ESb lymphoma cells with a retroviral expression vector containing the lacZ (bacterial beta-galactosidase) gene. This allowed single ESb-lacZ tumor cells to be detected in infiltrated target organs by means of X-Gal staining. Despite expression of the lacZ gene, the tumor cells were still tumorigenic, highly metastatic, unchanged in phenotype and therefore comparable to parental ESb cells. After spontaneous metastasis, whole-organ staining revealed metastatic foci at the surface of the liver. In histological liver sections, metastatic clusters and single dispersed tumor cells could be detected. In contrast to whole-organ staining, histological examination revealed scattered distribution of tumor cells throughout the organ, which was not evident with parental ESb cells. In addition, clusters with diffuse or dense (focal) appearance were found, in correlation with the whole-organ staining. Expression of the foreign lacZ gene allowed the metastatic spread of tumor cells to liver and spleen to be quantified approximately by FACS analysis. Furthermore, it was shown that the newly expressed beta-gal was expressed not only intercellularly but also at the cell ...Continue Reading

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Citations

May 3, 1996·International Journal of Cancer. Journal International Du Cancer·M HafnerD N Männel
Jun 1, 1996·Cancer Metastasis Reviews·V UmanskyV Schirrmacher
Jan 1, 1996·Breast Cancer Research and Treatment·S W McLeskeyF G Kern
Jul 27, 2011·Clinical & Experimental Metastasis·Florian SchelterAchim Krüger
Oct 29, 1998·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·L FjellbirkelandR Bjerkvig
Apr 28, 2006·Journal of Virology·Katja C ErlachJürgen Podlech
Mar 16, 1995·International Journal of Cancer. Journal International Du Cancer·P OroszD N Männel
Aug 19, 2014·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Bastian SeubertAchim Krüger
Sep 25, 2015·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Achim Krüger
Aug 25, 2004·Osteoarthritis and Cartilage·P UeblackerV Martinek
Nov 1, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Martin R WeihrauchHeribert Bohlen
Aug 12, 2003·Nature Medicine·Markus FeuererVolker Schirrmacher
Sep 5, 2008·Current Protocols in Cytometry·M Lorincz, M Roederer
Feb 2, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Matthias J E ArltBruno Fuchs
May 4, 2005·Cancer Research·Achim KrügerRafael Fridman
Mar 14, 2008·Molecular Cancer Research : MCR·Michael GergAchim Krüger
Feb 26, 2008·Molecular Cancer Research : MCR·Julia L ZhongDylan R Edwards

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