Scavenging of hydroxyl radicals but not of peroxynitrite by inhibitors and substrates of nitric oxide synthases

British Journal of Pharmacology
A RehmanB Halliwell

Abstract

1. The nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME) is widely used to study the role of NO. in physiological and pathological processes, including its role in the generation of the cytotoxic species peroxynitrite (ONOO-) and of reactive oxygen radicals such as hydroxyl (OH.). Often L-NAME is applied to tissues at mM concentrations. At such high concentrations, it might act as a free radical scavenger. A similar possibility might apply to the use of high levels of arginine to study the role of NO. in atherogenesis. 2. We therefore examined the rate of scavenging of OH. by L-NAME and found that L-NAME reacts more quickly with OH. than the established 'OH. scavenger' mannitol and the widely used 'OH. trap' salicylate. However, D-NAME can scavenge OH. at rates equal to L-NAME. Both L- and D-arginine were also good OH. scavengers, comparable in effectiveness to mannitol. 3. Neither L-NAME, D-NAME, L-arginine nor D-arginine was able to inhibit ONOO(-)-dependent nitration of tyrosine, suggesting that they are unlikely to be scavengers of ONOO(-)-derived nitrating species. 4. Neither L-NAME, D-NAME, L-arginine nor D-arginine was able to inhibit the inactivation of alpha 1-antiproteinase by ONOO-, suggesting...Continue Reading

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Citations

Sep 29, 2011·Journal of Physiology and Biochemistry·Przemysław SosnowskiRafał Jastak
Mar 5, 2004·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Santhanam A V Raghavan, Madhu Dikshit
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Jul 18, 2003·Journal of Neurotrauma·Eiichi SuehiroJohn T Povlishock
Oct 2, 2009·Journal of Clinical Biochemistry and Nutrition·Nobuyuki EndoYashige Kotake
Jul 10, 2008·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Marcela A AvilaDouglas S Dewitt
Apr 2, 2004·International Journal of Clinical Practice·H Hurairah, A Ferro
Aug 12, 2000·Redox Report : Communications in Free Radical Research·M K BarthwalM Dikshit
May 29, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·E M GoldingR M Bryan
Jan 10, 2003·The Japanese Journal of Physiology·Hitomi KawanamiTetsuya Izawa
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May 12, 2021·Journal of Cellular and Molecular Medicine·Ying ZhangZhirong Jiang
May 9, 2008·Journal of Agricultural and Food Chemistry·Carla SousaPaula B Andrade

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