Scavenging of nitric oxide by hemoglobin in the tunica media of porcine coronary arteries

Nitric Oxide : Biology and Chemistry
Alexander KollauBernd Mayer

Abstract

Scavenging of nitric oxide (NO) often interferes with studies on NO signaling in cell-free preparations. We observed that formation of cGMP by NO-stimulated purified soluble guanylate cyclase (sGC) was virtually abolished in the presence of cytosolic preparations of porcine coronary arteries, with the scavenging activity localized in the tunica media (smooth muscle layer). Electrochemical measurement of NO release from a donor compound and light absorbance spectroscopy showed that cytosolic preparations contained a reduced heme protein that scavenged NO. This protein, which reacted with anti-human hemoglobin antibodies, was efficiently removed from the preparations by haptoglobin affinity chromatography. The cleared cytosols showed only minor scavenging of NO according to electrochemical measurements and did not decrease cGMP formation by NO-stimulated sGC. In contrast, the column flow-through caused a nearly 2-fold increase of maximal sGC activity (from 33.1 ± 1.6 to 54.9 ± 2.2 μmol × min(-1) × mg(-1)). The proteins retained on the affinity column were identified as hemoglobin α and β subunits. The results indicate that hemoglobin, presumably derived from vasa vasorum erythrocytes, is present and scavenges NO in preparations o...Continue Reading

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Citations

Feb 10, 2019·Scientific Reports·Achini K VidanapathiranaPeter J Psaltis
Oct 20, 2020·Medicinal Research Reviews·Achini K VidanapathiranaStephen J Nicholls

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Methods Mentioned

BETA
PCA
Protein Assay
GTPase
immunoprecipitation
dot blots
dissection

Software Mentioned

SpectrumMill

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