SCFAs induce autophagy in intestinal epithelial cells and relieve colitis by stabilizing HIF-1α.

Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte
Chao ZhouHua Yang

Abstract

Hypoxia-inducible factor-1α (HIF-1α) is a critical regulator of barrier integrity during colonic mucosal injury. Previous works have shown that the absence of autophagy is implicated in the development of inflammatory bowel disease (IBD). Additionally, changes in bacterial profiles in the gut are intimately associated with IBD. Although HIF-1α, autophagy, microbiota, and their metabolites are all involved in the pathogenesis of IBD, their roles are not known. In this study, we investigated the relationship between HIF-1α and autophagy in healthy and inflammatory states using transgenic mice, colitis models, and cell culture models. We confirmed that the absence of intestinal epithelial HIF-1α changed the composition of the intestinal microbes and increased the susceptibility of mice to dextran sodium sulfate (DSS)-induced colitis. In addition, autophagy levels in the intestinal epithelial cells (IECs) were significantly reduced in IEC-specific HIF-1α-deficient (HIF-1α∆IEC) mice. Moreover, in the cell culture models, butyrate treatment significantly increased autophagy in HT29 cells under normal conditions, whereas butyrate had little effect on autophagy after HIF-1α ablation. Furthermore, in the DSS-induced colitis model, butyr...Continue Reading

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Citations

Apr 4, 2021·Journal of Clinical Medicine·Jorge OjedaPía M Vidal
Jun 26, 2021·Trends in Immunology·Laís P PralMarco A R Vinolo
Oct 19, 2021·Cell Biology International·Jiuheng YinHua Yang

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Methods Mentioned

BETA
gene knockout
IECs
IEC
PCRs
transmission electron microscopy
PCR
confocal microscopy
ubiquitination

Software Mentioned

GraphPad Prism

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