Schinus terebinthifolius scale-up countercurrent chromatography (Part I): High performance countercurrent chromatography fractionation of triterpene acids with off-line detection using atmospheric pressure chemical ionization mass spectrometry

Journal of Chromatography. a
Mariana Neves VieiraGerold Jerz

Abstract

'Countercurrent chromatography' (CCC) is an ideal technique for the recovery, purification and isolation of bioactive natural products, due to the liquid nature of the stationary phase, process predictability and the possibility of scale-up from analytical to preparative scale. In this work, a method developed for the fractionation of Schinus terebinthifolius Raddi berries dichloromethane extract was thoroughly optimized to achieve maximal throughput with minimal solvent and time consumption per gram of processed crude extract, using analytical, semi-preparative and preparative 'high performance countercurrent chromatography' (HPCCC) instruments. The method using the biphasic solvent system composed of n-heptane-ethyl acetate-methanol-water (6:1:6:1, v/v/v/v) was volumetrically scaled up to increase sample throughput up to 120 times, while maintaining separation efficiency and time. As a fast and specific detection alternative, the fractions collected from the CCC-separations were injected to an 'atmospheric pressure chemical ionization mass-spectrometer' (APCI-MS/MS) and reconstituted molecular weight MS-chromatograms of the APCI-ionizable compounds from S. terebinthifolius were obtained. This procedure led to the direct isola...Continue Reading

References

Apr 15, 2004·Phytochemistry·Mitsuko MakinoYasuo Fujimoto
Nov 5, 2005·Journal of Chromatography. a·A Marston, K Hostettmann
Mar 8, 2007·Journal of Chromatography. a·Philip WoodIan A Sutherland
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Feb 20, 2010·Journal of Separation Science·Fernanda das Neves Costa, Gilda Guimarães Leitão
Jan 1, 2008·Journal of Dietary Supplements·Lana Dvorkin-Camiel, Julia S Whelan

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Citations

Aug 6, 2016·Journal of Chromatography. a·Artak E Kostanyan, Andrey A Erastov
Sep 10, 2016·Journal of Chromatography. a·Fernanda das Neves CostaSvetlana Ignatova
Sep 9, 2016·Analytical Chemistry·Meng-Xi WuYin-Long Guo
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fernanda das Neves CostaSvetlana Ignatova

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