Abstract
To address the molecular mechanism of the anti-inflammation effects of schisandrin B (Sch B) in atherosclerosis, we examined injured HMEC-1, HBMEC, and HUVEC-12 cells induced by high glucose (HG). Western blot was performed to detect the levels of the proteins Hsp27, Noxa, TLR5, p-IκBα, and p-p65 in HG-induced cells, while ELISA was used to analyze the inflammatory cytokines TNF-α, IL-6, MCP-1, and IL-1β in cells with Hsp27 or Noxa stable expression. Overexpression of Hsp27 upregulated the inflammatory cytokines and the release of IκBα, promoted transportation of p65 into the nucleus, and lastly, affected the inflammation process, while Sch B counteracted the upregulation. In addition, the effect of Noxa overexpression, which is different from Hsp27 overexpression, was consistent with that of Sch B treatment. Sch B may inhibit the inflammatory cascade and alleviate the injury to HMEC-1, HBMEC, and HUEVC-12 cells caused by HG by regulating the Noxa/Hsp27/NF-κB signaling pathway.
References
Jun 28, 2003·The Journal of Biological Chemistry·Kyu-Jin ParkYoun Tae Kwak
Jun 15, 2004·Medical Hypotheses·Jian-Jun Li, Chun-Hong Fang
Jan 18, 2005·Trends in Biochemical Sciences·Patrick ViatourAlain Chariot
Apr 22, 2005·The New England Journal of Medicine·Göran K Hansson
Apr 22, 2006·Biogerontology·Po Yee ChiuKam Ming Ko
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Andrea Oeckinghaus, Sankar Ghosh
Apr 30, 2011·Circulation Research·Joseph W GordonLorrie A Kirshenbaum
Nov 22, 2011·Biochemical Pharmacology·Eun-Jung ParkJu-Hong Jeon
Aug 2, 2012·Cell Stress & Chaperones·Samira SalariEdward R O'Brien
Mar 15, 2013·Biochimica Et Biophysica Acta·Yadav WagleyLi-Na Wei
Sep 21, 2013·International Journal of Molecular Medicine·Monowar AzizYoshikazu Kinoshita
Nov 21, 2013·Biochimica Et Biophysica Acta·Rooban B NahomiRam H Nagaraj
Jul 1, 2014·Fitoterapia·Jung Nyeo ChunJu-Hong Jeon
Aug 9, 2015·Journal of Ethnopharmacology·Kyoung-Pil LeeDong-Soon Im
Jul 13, 2016·Mediators of Inflammation·Mei-Hua BaoHong-Hao Zhou
Aug 11, 2016·Frontiers in Immunology·Zarah BatulanEdward R O'Brien
Jan 14, 2017·Cell·Qian ZhangDavid Baltimore
Dec 17, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Guopei ZhengZhimin He
Jan 25, 2018·Mucosal Immunology·C ZhangY Tesfaigzi
Mar 10, 2018·Scientific Reports·Jee-Youn KimJaeho Cho
May 13, 2018·Particle and Fibre Toxicology·Bendik C BrinchmannJørn A Holme
May 16, 2018·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Yang YangYe Tian
May 23, 2018·Drug Design, Development and Therapy·Jisheng RanLidong Wu
Aug 2, 2018·Lipids in Health and Disease·Jiqu XuFenghong Huang
Citations
Aug 28, 2020·Frontiers in Pharmacology·Jia WangFeng Li
Nov 1, 2020·Journal of Cellular and Molecular Medicine·Jiao DuKai Sun
Dec 22, 2020·European Journal of Pharmacology·Yuan ZhouXiang Fan