Schisandrin B protects PC12 cells against oxidative stress of neurodegenerative diseases

Neuroreport
En-Ping JiangSen Wang

Abstract

Increasing evidence places Schisandrin B (Sch B) at an important position in nerve protection, indicating that Sch B might play a positive role in the therapy of neurodegenerative diseases. However, there is little information on it. Our studies showed that pretreatment with Sch B could reduce lactate dehydrogenase, malondialdehyde, and reactive oxygen species release and significantly increase the cell viability and the superoxide dismutase level. Sch B (10 μM) markedly inhibited cell apoptosis, whereas LY294002 (20 μM), a phosphatidylinositol-3 kinase inhibitor, blocked the antiapoptotic effect. More importantly, Sch B (10 μM) increased the phosphoprotein kinase B/protein kinase B (Akt) and B-cell lymphoma-2/Bcl-2 associated X protein ratios on preincubation with cells for 2 h, which was then inhibited by LY294002 (20 μM). Results indicate that Sch B can protect PC12 cells from apoptosis by activating the phosphatidylinositol-3 kinase/Akt signaling pathway and may emerge as a potential drug for neurodegenerative diseases.

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Citations

Sep 10, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yan JiangChang Liu
May 10, 2018·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Ying WuMei Tang
Apr 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Bing-You YangHai-Xue Kuang
Apr 8, 2020·Oxidative Medicine and Cellular Longevity·M I NasserPing Zhu
Oct 30, 2020·Neuropsychiatric Disease and Treatment·Jing Wang, Xingmao Wu
Jul 12, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Fatemeh AbbaszadehHaroon Khan

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Methods Mentioned

BETA
flow cytometry
MDA
protein assay

Software Mentioned

SPSS

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