Schisandrin B suppresses glioma cell metastasis mediated by inhibition of mTOR/MMP-9 signal pathway

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Yan JiangChang Liu

Abstract

Malignant glioma is the aggressive tumor in the brain and is characterized by high morbidity, high mortality. The main purpose of the present study was to investigate the therapeutic effects of Schisandrin B on glioma cells and preliminary explore the possible mechanism underlying anti-metastasis of Schisandrin B. Two glioma cell lines, U251 and U87, were used in present study. The ability of metastasis of glioma cells was evaluated using transwell migration assay and invasion assay. Expression of Akt, mTOR, MMP-2 and MMP-9 was determined using Western blotting. Antagonist or agonist was used to activated or inactivated signal molecules. Schisandrin B suppressed cell migration and invasion in manner of dose dependent as well as inhibited expression of p-Akt, p-mTOR and MMP-9. Activation of PI3K by 740Y-P treatment leaded to upregulation of p-Akt, mTOR and MMP-9; inactivation of mTOR by Rapamycin treatment inhibited expression MMP-9 while activation of mTOR by l-Leucine treatment enhanced MMP-9 expression in Schisandrin B incubated cells. Anti-migration and invasion action of Schisandrin B was also reversed by mTOR activation. Our findings demonstrate that Schisandrin B can suppress migration and invasion of glioma cell via PI3K...Continue Reading

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Citations

Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zhenfeng JiangZhiguo Lin
Jun 23, 2016·Oncotarget·Shao-Wei FengDueng-Yuan Hueng
Aug 1, 2019·Journal of Asian Natural Products Research·Ya-Jing LiZhan-Jun Dong
Jun 10, 2016·Journal of Neuro-oncology·Jai-Nien TungKun-Chung Chen
May 10, 2017·Neurochemical Research·Xue Tao QiAlbert Cheung Hoi Yu
Sep 27, 2020·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Efthalia AngelopoulouChristina Piperi
Dec 10, 2020·Drug Development and Industrial Pharmacy·Xiu-Ying LiQing-Shan Li
May 22, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shi-Lei TangWen-Zhong Hu

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