Schistosoma mansoni antigen Sm-p80: prophylactic efficacy using TLR4 agonist vaccine adjuvant glucopyranosyl lipid A-Alum in murine and non-human primate models

Journal of Investigative Medicine : the Official Publication of the American Federation for Clinical Research
Weidong ZhangAfzal A Siddiqui

Abstract

Sm-p80, the large subunit of Schistosoma mansoni calpain, is a leading candidate for a schistosomiasis vaccine. The prophylactic and antifecundity efficacy of Sm-p80 has been tested in three animal models (mouse, hamster and baboon) using a multitude of vaccine formulations and approaches. In our continual effort to enhance the vaccine efficacy, in this study, we have utilized the adjuvant, synthetic hexa-acylated lipid A derivative, glucopyranosyl lipid A (GLA) formulated in aluminum (GLA-Alum) with recombinant Sm-p80. The rSm-p80+GLA-Alum immunization regimen provided 33.33%-53.13% reduction in worm burden in the mouse model and 38% worm burden reduction in vaccinated baboons. Robust Sm-p80-specific immunoglobulin (Ig)G, IgG1, IgG2a and IgM responses were observed in all immunized animals. The rSm-p80+GLA-Alum coadministration induced a mix of T-helper (Th) cells (Th1, Th2 and Th17) responses as determined via the release of interleukin (IL)-2, IL-4, IL-18, IL-21, IL-22 and interferon-γ.

References

Feb 1, 1977·Experimental Parasitology·P M KnopfJ A Reasoner
Jan 23, 1999·Journal of Immunological Methods·A FreyD Zurakowski
Aug 22, 2000·Parasitology Today·R Corrêa-OliveiraG Gazzinelli
Jul 3, 2002·Nature Reviews. Immunology·Edward J Pearce, Andrew S MacDonald
Mar 24, 2004·Nature Reviews. Microbiology·L ChitsuloD Engels
Oct 14, 2004·Immunology and Cell Biology·Erik B Lindblad
Sep 26, 2006·Lancet·Bruno GryseelsLuc Kestens
Jun 15, 2007·Nature Reviews. Microbiology·Bruno Guy
Jan 19, 2008·Clinical Microbiology Reviews·Donald P McManus, Alex Loukas
Sep 30, 2008·PLoS Neglected Tropical Diseases·R Alan WilsonPatricia S Coulson
Mar 10, 2009·Human Vaccines·Hemamali J WarshakoonSunil A David
Sep 1, 2009·Trends in Parasitology·R Alan Wilson, Patricia S Coulson
Jul 16, 2010·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Gul AhmadAfzal A Siddiqui
Aug 13, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mark T OrrRhea N Coler
Jan 10, 2014·The American Journal of Tropical Medicine and Hygiene·Annie X MoLance Gordon
Feb 11, 2014·Current Protocols in Immunology·Matthew S TuckerYung-San Liang
Apr 5, 2014·Lancet·Daniel G ColleyCharles H King
Feb 19, 2016·Parasites & Vectors·R Alan WilsonWilliam Castro-Borges
Nov 21, 2016·Trends in Parasitology·Afzal A Siddiqui, Sabrina Z Siddiqui

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Citations

Jun 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Syed Amir AshrafSibte Hadi
May 3, 2019·Oxidative Medicine and Cellular Longevity·Tingli SunPeilong Ma
Dec 24, 2019·Frontiers in Cellular and Infection Microbiology·Shahram Solaymani-MohammadiSteven M Singer
Mar 18, 2020·Journal of Advanced Research·Arif Jamal SiddiquiWhitni Redman
May 4, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Aya Al-NaseriNoha Mahana

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Methods Mentioned

BETA
size exclusion chromatography
ELISA
density gradient centrifugation
reverse transcription-PCR
Flow

Software Mentioned

FlowJo
Quantity One Programme
Systat
CellQuset
Sigma Plot
Quantity One

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