Schistosoma mansoni arginase shares functional similarities with human orthologs but depends upon disulphide bridges for enzymatic activity.

International Journal for Parasitology
Jennifer M FitzpatrickMatthias Hesse

Abstract

Schistosome helminths constitute a major health risk for the human population in many tropical areas. We demonstrate for the first time that several developmental stages of the human parasite Schistosoma mansoni express arginase, which is responsible for the hydrolysis of l-arginine to l-ornithine and urea. Arginase activity by alternatively activated macrophages is an essential component of the mammalian host response in schistosomiasis. However, it has not been previously shown that the parasite also expresses arginase when it is in contact with the mammalian host. After cloning and sequencing the cDNA encoding the parasite enzyme, we found that many structural features of human arginase are well conserved in the parasite ortholog. Subsequently, we discovered that S. mansoni arginase shares many similar molecular, biochemical and functional properties with both human arginase isoforms. Nevertheless, our data also reveal striking differences between human and schistosome arginase. Particularly, we found evidence that schistosome arginase activity depends upon disulphide bonds by cysteines, in contrast to human arginase. In conclusion, we report that S. mansoni arginase is well adapted to the physiological conditions that exist...Continue Reading

References

May 1, 1989·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M TakiguchiM Mori
Mar 28, 1986·Biochimica Et Biophysica Acta·N Carvajal, S D Cederbaum
Jan 1, 1974·Advances in Parasitology·M A Stirewalt
Apr 12, 1966·Biochimica Et Biophysica Acta·J MoraL F Bojalil
Sep 24, 1982·Science·P H YanceyG N Somero
May 1, 1994·Comparative Biochemistry and Physiology. Pharmacology, Toxicology and Endocrinology·I P OswaldS L James
Sep 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·N CarvajalM Salas
Sep 1, 1994·Archives Internationales De Physiologie, De Biochimie Et De Biophysique·J M FuentesG Soler
May 1, 1994·The International Journal of Biochemistry·J M FuentesG Soler
Jul 1, 1994·Journal of Molecular Evolution·C A Ouzounis, N C Kyrpides
May 1, 1996·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C P JenkinsonS D Cederbaum
Oct 10, 1996·Nature·Z F KanyoD W Christianson
Mar 21, 1998·Biochimica Et Biophysica Acta·J PerozichS M Morris
Jan 22, 2000·Journal of Inorganic Biochemistry·N CarvajalM Fuentes
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·M A Martí-RenomA Sali
Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R RutschmanP J Murray
May 24, 2001·Archives of Biochemistry and Biophysics·D M ColleluoriD E Ash
Aug 2, 2001·Biochemistry·D M Colleluori, D E Ash
Aug 29, 2001·Bioinformatics·J P Huelsenbeck, F Ronquist
Nov 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M HesseT A Wynn
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·A P GobertK T Wilson

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Citations

Nov 4, 2009·PLoS Neglected Tropical Diseases·Jennifer M FitzpatrickKarl F Hoffmann
Jul 20, 2010·Immunological Reviews·Ursula Grohmann, Vincenzo Bronte
May 7, 2020·PLoS Neglected Tropical Diseases·Arporn WangwiwatsinMatthew Berriman
Feb 8, 2021·International Journal of Biological Macromolecules·Iara Aimê CardosoMaria Cristina Nonato

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