Schistosoma mansoni: detection of circulating antigens in murine schistosomiasis by antigen-capture sandwich ELISA using a monoclonal antibody

Experimental Parasitology
I S BarsoumK A Kamal

Abstract

A monoclonal antibody (MAb) 5H11/B1 that reacts with a repeating epitope on an excretory-secretory (E + S) antigen of adult worms of Schistosoma mansoni was used in the detection of circulating antigen (CA) in sera from S. mansoni-infected mice using an antigen-capture sandwich ELISA. Trichloroacetic acid (TCA) pretreatment of sera from mice infected for 8 or 16 weeks precipitated immune complexes and/or dissociated CA and allowed its detection. Sera obtained 8 weeks after infection contained high levels of CA. Upon treatment with praziquantel (100 mg/kg body wt), this level was significantly less within 1 week. A strong correlation was found between the worm count determined by perfusion and the level of antigenemia detected by the 5H11/B1 assay in light and heavy infection (r = 0.80). Based on the results of both TCA pretreatment and sodium periodate treatment, the 5H11/B1 sandwich ELISA assay detects a repeating carbohydrate epitope on an E + S antigen. This system appears to be a sensitive assay for the detection of schistosomal antigenemia in murine schistosomiasis. Studies on the detection of antigenemia in human schistosomiasis using this assay are in progress.

References

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·W E StewartK Cantell
Oct 1, 1976·Experimental Parasitology·A M DeelderE H van Meerbeke
Jan 1, 1989·The American Journal of Tropical Medicine and Hygiene·A M DeelderA M Polderman
May 1, 1989·Transactions of the Royal Society of Tropical Medicine and Hygiene·N de JongeA M Deelder
May 1, 1988·The American Journal of Tropical Medicine and Hygiene·G J WeilT K Ghosh
Jan 1, 1986·The American Journal of Tropical Medicine and Hygiene·A F TawfikD G Colley
Jan 1, 1988·Transactions of the Royal Society of Tropical Medicine and Hygiene·N De JongeA M Deelder
Mar 1, 1988·The American Journal of Tropical Medicine and Hygiene·M N LundeE A Ottesen
Jun 28, 1988·Journal of Immunological Methods·M García-GonzálezP Pouletty
Nov 1, 1974·The American Journal of Tropical Medicine and Hygiene·T E Nash
Sep 1, 1967·The American Journal of Tropical Medicine and Hygiene·W L Berggren, T H Weller
Nov 1, 1982·The American Journal of Tropical Medicine and Hygiene·J K Weltman
Dec 1, 1981·Experimental Parasitology·J P RotmansA M Deelder

❮ Previous
Next ❯

Citations

Jan 1, 1994·International Journal of Antimicrobial Agents·V Kumar, B Gryseels
Aug 6, 1998·Transactions of the Royal Society of Tropical Medicine and Hygiene·L Van LieshoutA M Deelder
Feb 2, 2011·PLoS Neglected Tropical Diseases·Hillary L ShaneW Evan Secor
Jul 1, 2011·Tropical Parasitology·Subhash Chandra Parija, Ar Gireesh
Sep 7, 2000·Transactions of the Royal Society of Tropical Medicine and Hygiene·M M SantosA Rabello
Jan 22, 2003·Tropical Medicine & International Health : TM & IH·Yong-Long LiAndreas Ruppel
Feb 25, 1999·Memórias do Instituto Oswaldo Cruz·Y Pereira e SilvaA Rabello
Aug 1, 2015·Clinical Microbiology Reviews·Kosala G A D WeerakoonDonald P McManus

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.