PMID: 2493503Mar 15, 1989Paper

Schistosoma mansoni: the host immune response to egg antigens. I. Partial characterization of cellular and humoral responses to pI fractions of soluble egg antigens

The Journal of Immunology : Official Journal of the American Association of Immunologists
D A HarnM J Stadecker

Abstract

Soluble egg Ag (SEA) were separated according to charge by agarose-IEF (A-IEF) in order to partially characterize those antigenic determinants that may elicit the granulomatous response in schistosomiasis mansoni. Coomassie blue-stained A-IEF gels of SEA showed that this technique was able to resolve multiple isobands. A-IEF gels were sliced into 18 or 36 fractions from anode to cathode, and the SEA components from the isoelectric point (pI) fractions were eluted from gels in a manner that allowed for their direct utilization in both cellular and in humoral immunoassays. Only Ag in two distinct pI ranges consistently elicited lymphoproliferative responses, with the major stimulatory fractions being in the acid (3.5 to 5.0) range. Lymphocytes from mice infected for 10 wk had higher proliferative responses to both SEA and fractionated Ag in this acid pI range when compared to lymphocytes from mice infected for 25 wk; however, the latter had an enhanced response to Ag with a pI range of 6.2 to 6.4. In contrast to the cellular responses, the corresponding sera from these mice, or from schistosome-infected patients, recognized Ag in all pI fractions as determined by ELISA. However, although protective and nonprotective mAb (which re...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.