Schizandrin protects H9c2 cells against lipopolysaccharide-induced injury by downregulating Smad3

Journal of Biochemical and Molecular Toxicology
Xuehua ZhangShan Cong

Abstract

Schizandrin is a major bioactive constituent of Schisandra chinensis (Turcz.) Baill with antioxidant and anti-inflammatory properties. The objective of this study was to explore the potential effects of schizandrin on a cell model of myocarditis. The H9c2 cells were treated with schizandrin alone or in combination with lipopolysaccharide (LPS), after which, cell survival, migration, and the release of inflammatory cytokines were assessed. Moreover, downstream effectors and signaling pathways were studied to reveal the possible underlying mechanism. As a result, LPS stimulation induced significant cell damage as cell viability was repressed and the apoptosis was induced. In the meantime, LPS promoted the release of proinflammatory cytokines including interleukin 1β (IL-1β), IL-8, IL-6, and tumor necrosis factor (TNF-α) while repressing the release of the anti-inflammatory cytokine IL-10. Schizandrin could promote H9c2 cell migration and long-term treatment (7 days) enhanced cell viability. More interestingly, pretreatment with schizandrin attenuated LPS-induced cell loss and inflammatory response. Besides this, Smad3 was a downstream effector of schizandrin. The beneficial effects of schizandrin on the H9c2 cells were attenuated...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 16, 2008·European Journal of Pharmacology·Lian Yu GuoYeong Shik Kim
May 9, 2009·Nature Medicine·Peter A Ward
Jan 30, 2010·Progress in Cardiovascular Diseases·Lori A Blauwet, Leslie T Cooper
Feb 19, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ting ZhaoXiangyang Wu
Oct 25, 2013·Journal of Applied Toxicology : JAT·Valérian BunelCaroline Stévigny
Mar 18, 2017·American Journal of Respiratory Cell and Molecular Biology·Xiaoju TangFengming Luo
Sep 5, 2017·Oncogene·P ChandrasingheJ Warusavitarne
Mar 9, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Haifang ZhangLijuan Zhang
Mar 30, 2018·Archives of Medical Science : AMS·Sohaib TariqWilbert S Aronow

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Citations

Jun 10, 2020·The Journal of Organic Chemistry·Wu ZhangWanxiang Zhao

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