Schizosaccharomyces pombe has a novel eukaryotic initiation factor 4F complex containing a cap-binding protein with the human eIF4E C-terminal motif KSGST.

The Journal of Biological Chemistry
Marina PtushkinaJ E McCarthy

Abstract

Genetic and biochemical analyses were performed on the cytoplasmic cap-binding complex (eukaryotic initiation factor (eIF) 4F) of Schizosaccharomyces pombe. Genomic and cDNA sequencing of the S. pombe gene (tif1) encoding the cap-binding component eIF4E revealed the presence of two introns in a reading frame of 219 codons. The encoded sequence of 218 amino acids shows a greater degree of identity to the mammalian eIF4E sequence than does its counterpart from Saccharomyces cerevisiae. In particular, unlike its S. cerevisiae counterpart, S.pombe eIF4E has a C-terminal Ser209 within the motif KSGST that is a site of phosphorylation in hamster and rabbit eIF4E. Of relevance to its potential regulatory role, eIF4E was found to be encoded by an mRNA with a six-nucleotide leader and to be of low abundance in vivo. Cross-linking experiments identified S. pombe eIF4E as the major cap-binding protein while a further protein, p36, also showed cap-dependent binding. eIF4A was not associated with the cap-binding complex. While S. pombe eIF4E was shown capable of binding S. cerevisiae p20, an equivalent protein was absent from the eIF4F complex isolated from S. pombe cells. S. pombe 4F therefore shows a remarkable combination of structural a...Continue Reading

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Citations

Nov 20, 2001·Nucleic Acids Research·M PtushkinaJ E McCarthy
May 22, 2004·European Journal of Biochemistry·Bhavesh JoshiRosemary Jagus
Feb 18, 2004·EMBO Reports·Marina PtushkinaJohn E G McCarthy
Apr 5, 2005·Mechanisms of Development·Greco HernándezRolando Rivera-Pomar
Jun 1, 2005·Mechanisms of Development·Greco Hernández, Paula Vazquez-Pianzola
Jul 17, 2009·Critical Reviews in Biochemistry and Molecular Biology·Katrien Van Der KelenLieven De Veylder
Apr 5, 2020·International Journal of Molecular Sciences·Daniela Ross-Kaschitza, Michael Altmann
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·J E McCarthy

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