Schizosaccharomyces pombe possesses two paralogous valyl-tRNA synthetase genes of mitochondrial origin

Molecular Biology and Evolution
Wen-Chih ChiuChien-Chia Wang

Abstract

Previous studies showed that VAS1 of Saccharomyces cerevisiae encodes both cytosolic and mitochondrial forms of valyl-tRNA synthetase (ValRS) through alternative initiation of translation. We show herein that except for Schizosaccharomyces pombe, all yeast species studied contained a single ValRS gene encoding both forms, and all of the mature protein forms deduced from those genes possessed an N-terminal appended domain (Ad) that was absent from their bacterial relatives. In contrast, S. pombe contained two distinct nuclear ValRS genes, one encoding the mitochondrial form and the other its cytosolic counterpart. Although the cytosolic form closely resembles other yeast ValRS sequences (approximately 60% identity), the mitochondrial form exhibits significant divergence from others (approximately 35% identity). Both genes are active and essential for the survival of the yeast. Most conspicuously, the mitochondrial form lacks the characteristic Ad. A phylogenetic analysis further suggested that both forms of S. pombe ValRS are of mitochondrial origin, and the mitochondrial form is ancestral to the cytoplasmic form.

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Jan 25, 2006·The Journal of Biological Chemistry·Kuang-Jung ChangChien-Chia Wang
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Jul 4, 2009·The Journal of Biological Chemistry·Wen-Chih ChiuChien-Chia Wang

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Citations

Sep 1, 2011·ACS Chemical Biology·Shun-Jia ChenChien-Chia Wang
Jul 24, 2012·Nucleic Acids Research·Chih-Chi LiaoChien-Chia Wang
Feb 17, 2015·Fungal Genetics and Biology : FG & B·Chin-I ChienChien-Chia Wang
Mar 23, 2017·Cellular and Molecular Life Sciences : CMLS·Yi-Hsueh LeeChien-Chia Wang

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