Schwann cell durotaxis can be guided by physiologically relevant stiffness gradients

Biomaterials Research
Elisabeth B EvansDiane Hoffman-Kim

Abstract

Successful nerve regeneration depends upon directed migration of morphologically specialized repair state Schwann cells across a nerve defect. Although several groups have studied directed migration of Schwann cells in response to chemical or topographic cues, the current understanding of how the mechanical environment influences migration remains largely understudied and incomplete. Therefore, the focus of this study was to evaluate Schwann cell migration and morphodynamics in the presence of stiffness gradients, which revealed that Schwann cells can follow extracellular gradients of increasing stiffness, in a form of directed migration termed durotaxis. Polyacrylamide substrates were fabricated to mimic the range of stiffness found in peripheral nerve tissue. We assessed Schwann cell response to substrates that were either mechanically uniform or embedded with a shallow or steep stiffness gradient, respectively corresponding to the mechanical niche present during either the fluid phase or subsequent matrix phase of the peripheral nerve regeneration process. We examined cell migration (velocity and directionality) and morphology (elongation, spread area, nuclear aspect ratio, and cell process dynamics). We also characterized t...Continue Reading

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Citations

Feb 15, 2020·The Journal of Pathology·Savvas Nikolaou, Laura M Machesky
Dec 4, 2019·Advanced Healthcare Materials·Céline KayalJames B Phillips
Feb 2, 2020·Biomechanics and Modeling in Mechanobiology·Gabriel Santos RosalemLibardo Andrés González-Torres
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Oct 4, 2020·Bioengineering·Gregory ReidMichela Pozzobon
Dec 9, 2020·Developmental Cell·Adam Shellard, Roberto Mayor
Apr 3, 2021·Physical Review Letters·Hadrien OliveriAlain Goriely
Apr 4, 2021·The FEBS Journal·Jaime A EspinaElias H Barriga
Jun 3, 2021·International Journal of Molecular Sciences·Zhenyuan XuGreg M Harris
Jun 12, 2021·Frontiers in Bioengineering and Biotechnology·Rebecca PowellJames B Phillips
Jul 20, 2021·Seminars in Cell & Developmental Biology·Deannah G BlackleyAparna Ratheesh
Sep 2, 2021·Acta Biomaterialia·Pallavi Uday ShirkeAbhijit Majumder

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Methods Mentioned

BETA
scanning electron microscopy
rheometry

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