Schwann Cell Transplantation Subdues the Pro-Inflammatory Innate Immune Cell Response after Spinal Cord Injury

International Journal of Molecular Sciences
Damien D PearseMousumi Ghosh

Abstract

The transplantation of Schwann cells (SCs) has been shown to provide tissue preservation and support axon growth and remyelination as well as improve functional recovery across a diverse range of experimental spinal cord injury (SCI) paradigms. The autologous use of SCs has progressed to Phase 1 SCI clinical trials in humans where their use has been shown to be both feasible and safe. The contribution of immune modulation to the protective and reparative actions of SCs within the injured spinal cord remains largely unknown. In the current investigation, the ability of SC transplants to alter the innate immune response after contusive SCI in the rat was examined. SCs were intraspinally transplanted into the lesion site at 1 week following a thoracic (T8) contusive SCI. Multicolor flow cytometry and immunohistochemical analysis of specific phenotypic markers of pro- and anti-inflammatory microglia and macrophages as well as cytokines at 1 week after SC transplantation was employed. The introduction of SCs significantly attenuated the numbers of cluster of differentiation molecule 11B (CD11b)⁺, cluster of differentiation molecule 68 (CD68)⁺, and ionized calcium-binding adapter molecule 1 (Iba1)⁺ immune cells within the lesion impl...Continue Reading

References

Jun 1, 1992·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·V H Perry, M C Brown
Jan 1, 1992·Journal of Neurotrauma·J A Gruner
Apr 30, 1998·Journal of the Neurological Sciences·L J MoriartyR B Borgens
Sep 22, 2001·Journal of Neurotrauma·P G PopovichC C Whitacre
May 25, 2004·Nature Medicine·Damien D PearseMary Bartlett Bunge
Mar 8, 2007·Annual Review of Neuroscience·Zu-Lin ChenSidney Strickland
Apr 8, 2009·The Journal of Comparative Neurology·Thomas Pang LoDamien Daniel Pearse
Oct 30, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kristina A KigerlPhillip G Popovich
Sep 1, 2011·Journal of Neuroinflammation·Andrew D GaudetMatt S Ramer
Sep 18, 2012·Biomaterials·Nassir MokarramRavi V Bellamkonda
Oct 23, 2012·Nature Neuroscience·Kira I MosherTony Wyss-Coray
Feb 7, 2013·Frontiers in Cellular Neuroscience·Audrey KarperienHerbert F Jelinek
Mar 9, 2013·Cell Death & Disease·K KobayashiK Kadomatsu
Jan 11, 2014·Experimental & Molecular Medicine·Dong-Im ChoYoungkeun Ahn
Jun 27, 2014·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Elisa R ZanierMaria-Grazia De Simoni
Nov 5, 2014·Spinal Cord·D J Allison, D S Ditor
Dec 3, 2014·Scientific Reports·Wing Yip Tam, Chi Him Eddie Ma
Apr 29, 2015·Mediators of Inflammation·Bruno Siqueira MiettoAna Maria Blanco Martinez
Aug 11, 2015·Stem Cells International·Guoping ZhengJianguo Xu
Dec 25, 2015·PloS One·Kyle A JablonskiMireia Guerau-de-Arellano
Feb 13, 2016·The Journal of Physiology·K R Jessen, R Mirsky
Mar 17, 2016·Neural Regeneration Research·Jo Anne Stratton, Prajay T Shah
Feb 23, 2017·Journal of Neurotrauma·Kim D AndersonAllan D Levi
Apr 30, 2017·Results and Problems in Cell Differentiation·Patricia Luz-CrawfordFarida Djouad

❮ Previous
Next ❯

Citations

Feb 15, 2020·Journal of Neurotrauma·Aaron D GilmourJames A St John
Dec 12, 2019·Frontiers in Bioengineering and Biotechnology·Cristiana R CarvalhoRui L Reis
May 8, 2020·Journal of Neuroinflammation·Thomas GrüterKalliopi Pitarokoili
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Lynn NazarethJenny Ekberg
Jul 29, 2021·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Chengfang TanSiqin Huang

❮ Previous
Next ❯

Methods Mentioned

BETA
Flow cytometry
mechanical
enzymatic dissociation

Software Mentioned

FACSDiva
SigmaStat
Image J
Graphpad

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here