Schwann cells co-cultured with stimulated T cells and antigen express major histocompatibility complex (MHC) class II determinants without interferon-gamma pretreatment: synergistic effects of interferon-gamma and tumor necrosis factor on MHC class II induction

European Journal of Immunology
A KingstonR Mirsky

Abstract

Schwann cells (SC) do not express major histocompatibility complex (MHC) class II antigens under normal culture conditions. SC can, however, be induced in vitro to express MHC class II molecules by exposure to high concentrations of interferon-gamma (IFN-gamma) and can present antigens to antigen-specific T cell lines. In the present study immunohistochemical labeling showed that most SC (greater than 90%) prepared from rat neonatal sciatic nerves expressed MHC class II molecules when cultured together with mycobacterial antigen and T cells, and as a consequence were able to function as antigen-presenting cells in lymphoproliferation assays, without requiring pretreatment with IFN-gamma. Antigen or T cells alone were ineffective in stimulating MHC class II expression and induction of class II molecules was MHC restricted, requiring the presence of syngeneic T cells. Addition of monoclonal antibody DB1, directed against IFN-gamma to co-cultures of SC and T lymphocytes stimulated with antigen, prevented the induction of MHC class II antigen on SC. When SC were incubated with recombinant (r)IFN-gamma alone, up to 50% of SC showed positive labeling for MHC class II antigen. This level of expression was enhanced to greater than 80% ...Continue Reading

References

Jun 1, 1986·The Journal of General Virology·P H van der MeideH Schellekens
Jul 1, 1987·European Journal of Immunology·G J Dougherty, N Hogg
Dec 1, 1987·Immunological Reviews·G M Edelman
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg
Mar 26, 1974·Acta Neuropathologica·J Boddingius
Oct 31, 1983·Neuroscience Letters·M R HirschC Goridis
Aug 23, 1984·Nature·G H WongJ W Schrader
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R NoelleE S Vitetta

❮ Previous
Next ❯

Citations

Dec 1, 1989·Journal of Neuroscience Research·P A EcclestonR Mirsky
Feb 1, 1995·Muscle & Nerve·H P HartungK V Toyka
Jan 1, 1996·Springer Seminars in Immunopathology·H P HartungG Giegerich
Feb 1, 1997·Molecular Neurobiology·S Y Fu, T Gordon
Jan 1, 1991·Immunologic Research·D R Johnson, J S Pober
Oct 1, 1991·Journal of Neuroimmunology·K BergsteinsdottirK R Jessen
Jun 1, 1994·Progress in Neurobiology·P J EvansS E Mackinnon
Dec 1, 1999·Developmental and Comparative Immunology·S RautenschleinJ M Sharma
Mar 10, 2001·Progress in Neurobiology·R KieferH P Hartung
Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K BergsteindottirR Mirsky
Apr 1, 2014·Journal of Neuroinflammation·Mark StettnerBernd C Kieseier
Jul 1, 1997·Journal of Neuroimmunology·O Lilje, P J Armati
Jun 1, 1993·Research in Immunology·S K Parida, G E Grau
Apr 1, 1997·Multiple Sclerosis : Clinical and Laboratory Research·A Baron-Van EvercoorenR Liblau
Oct 12, 2001·Glia·T BennN Scolding
Sep 30, 1999·Environmental Health Perspectives·H A El-FawalM Y Shamy
Sep 14, 2006·Current Opinion in Neurology·Bernd C KieseierHeinz Wiendl
Sep 8, 2007·Muscle & Nerve·Gerd Meyer zu HörsteBernd C Kieseier
Apr 18, 2014·Journal of Clinical Immunology·Nevena TzekovaPatrick Küry
Nov 5, 2019·Cancer Immunology, Immunotherapy : CII·Sophia H ZhangYuri L Bunimovich
Nov 15, 1990·International Journal of Cancer. Journal International Du Cancer·G MechtersheimerP Möller
Apr 29, 1999·Brain Pathology·R GoldH P Hartung
Dec 2, 1999·Immunologic Research·L GinaldiD Quaglino
May 9, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·E SpieringsT H Ottenhoff
Dec 22, 1999·Experimental and Molecular Pathology·O Lilje, P J Armati

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.