PMID: 9419009Jan 7, 1998Paper

Schwann cells modulate sodium channel expression in spinal sensory neurons in vitro

Glia
A W HinsonS G Waxman

Abstract

In order to study the factors that govern the expression of sodium channel alpha-, beta1- and beta2-subunits, the influence that Schwann cells (SC) exert in the expression of sodium channels in DRG neurons was examined with in situ hybridization, immunocytochemistry, and patch clamp recording. The expression of sodium channel alpha-, beta1-, and beta2-subunit mRNAs in DRG neurons isolated from E15 rats cultured in defined medium in the absence (control) or presence of SC, or in SC-conditioned medium, was examined with isoform-specific riboprobes for sodium channel alpha-subunits I, II, III, NaG, Na6, hNE/PN1, SNS, and beta1- and beta2-subunits. DRG neurons cultured in the presence of SC displayed a significant (P < 0.05) increase in the hybridization signal for NaG, Na6, SNS, and Na beta2 mRNAs in comparison to control DRG neurons. In contrast, in SC-conditioned medium, only the hybridization signal for SNS mRNA was significantly increased. The upregulation of sodium channel mRNAs in DRG neurons co-cultured with SC was paralleled by an increase in sodium channel immunoreactivity of these cells. An increase in the mean sodium current density in DRG neurons in the presence of SC was also observed. These results demonstrate that a...Continue Reading

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Citations

Jun 17, 1999·Lancet·C J Woolf, R J Mannion
Aug 11, 1999·Trends in Neurosciences·K R Jessen, R Mirsky
May 15, 2002·Brain Research. Molecular Brain Research·J A BlackS G Waxman
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Aug 5, 2003·Journal of Neuroscience Research·Matthew N RasbandJames S Trimmer
Feb 14, 2004·Molecular and Cellular Neurosciences·Jochen C UlzheimerRudolf Martini
Jul 1, 1997·Current Biology : CB·S G Waxman

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