Scientific validation of the antimicrobial and antiproliferative potential of Clerodendrum serratum (L.) Moon, its phytoconstituents and their biosafety by acute oral toxicity study.

Drug and Chemical Toxicology
Himadri MahajanJatinder Singh

Abstract

The screening of aqueous extract of Clerodendrum serratum revealed its broad-spectrum antimicrobial potential against Gram positive, Gram negative bacteria, and yeast. Optimizing the extraction strategies, revealed 15% concentration of aqueous extract prepared at 40 °C by extracting for 40 min, as optimum parameters and its statistical optimization by Box-Behnken design led to 1.16-1.35 folds enhancement in activity. Organic solvent extraction further improved the activity where methanol proved to be the best organic extractant which was effective against all the 13 pathogens tested with inhibition zone ranging from 14 to 32 mm. Minimum Inhibitory Concentration (MIC) study endorsed the methanolic extract to be the best organic extractant, as it showed the lowest MIC (0.5-10 mg/ml) in comparison to aqueous extract (1-10 mg/ml) as well as Partially Purified Phytoconstituents i.e., flavonoids (1-5 mg/ml), diterpenes (5-10 mg/ml) and cardiac glycosides (5-10 mg/ml). All these were found to be biosafe in both In-vitro (Ames and MTT assay) and In-vivo toxicity studies. Acute oral toxicity testing of flavonoids (2000 mg/ml) on Wistar rats did not reveal any significant change in relative organ weight, biochemical, hematological parame...Continue Reading

References

Jun 6, 2000·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·H Tsuchiya, M Iinuma
Apr 24, 2003·Medicinal Research Reviews·Wenying RenLi Zhang
Jun 14, 2003·Biochemical and Biophysical Research Communications·Andreja PlaperRoman Jerala
Aug 3, 2006·Medical Mycology·Claudete R PaulaFlavio A C Vaz
Nov 28, 2006·Animal Biotechnology·H HarbottleD G White
Aug 7, 2009·BMC Complementary and Alternative Medicine·Gurinder J Kaur, Daljit S Arora
Jul 31, 2010·Nanoscale Research Letters·Aliaksandra RakovichAlexander Eychmüller
Jun 28, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Subramanion L JothySreenivasan Sasidharan
Jul 11, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Thially Braga GonçalvesAparecida T Nagao-Dias
Sep 6, 2017·Applied Biochemistry and Biotechnology·D S Arora, Himadri Mahajan
Jan 13, 2018·Journal of Traditional and Complementary Medicine·Jin-Hui WangMao-Xing Li
Jul 19, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mehdi Sharifi-RadJavad Sharifi-Rad
Jul 25, 2018·Phytotherapy Research : PTR·Mehdi Sharifi-RadJavad Sharifi-Rad

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