PMID: 9170624Apr 1, 1997Paper

Scintigraphy of acute inflammatory lesions in rats with radiolabelled recombinant human interleukin-8

Nuclear Medicine Communications
R V HayM D Gross

Abstract

We compared 125I-labelled recombinant human interleukin-8 (125I-IL-8) with 111In-labelled human leukocytes (111In-WBC) and 67Ga-citrate for scintigraphic depiction of acute sterile inflammatory lesions in rats. Radioiodination of IL-8 was catalysed by chloramine-T, and human leukocytes were radiolabelled with 111In-oxine. Inflammatory lesions were induced in male rats by subcutaneous injection of 2% carrageenan suspension into their left hindlimbs. Twenty-four hours later, each rat received 1.8-3.7 MBq (50-100 microCi) of a single agent by intravenous injection. Sequential whole-body scintigrams were obtained between 0 and 96 h post-injection. Activities in the lesion-bearing and control hindlimbs were expressed as regional percent injected activity corrected for physical decay (%IA) by reference to concurrently imaged standards, and for 125I-IL-8 by direct tissue counting at necropsy as well. 125I-IL-8 displayed appropriate electrophoretic mobility, retained chemotactic and high-affinity receptor-binding activity in vitro, and exhibited exponentially decreasing activity in most tissues beginning shortly after intravenous injection. Scintigrams showed asymmetrically increased activity in the lesion-bearing hindlimb for all thre...Continue Reading

Citations

Nov 1, 1995·European Journal of Nuclear Medicine·C J van der LakenF H Corstens
Apr 9, 2010·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Mauro LiberatoreDomenico Rubello
Jun 8, 2012·International Journal of Peptides·Muammad Saeed AkhtarAbubaker Shahid
Oct 12, 2007·Future Microbiology·Ronald C WalkerDominique Delbeke
Aug 6, 2000·Seminars in Nuclear Medicine·O C BoermanF H Corstens
Oct 23, 2004·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·J K AmarteyR S Parhar
Oct 6, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rick V HayGeorge F Vande Woude
May 15, 2013·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Alexander GrabnerStefan Reuter

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