Scleral matrix metalloproteinases, serine proteinase activity and hydrational capacity are increased in myopia induced by retinal image degradation

Experimental Eye Research
B E JonesG J Chader

Abstract

In the avian model of myopia, retinal image degradation quickly leads to ocular enlargement. We now give evidence that regionally specific changes in ocular size are correlated with both biomechanical indices of scleral remodeling, e.g. hydration capacity and with biochemical changes in proteinase activities. The latter include a 72 kDa matrix metalloproteinase (putatively MMP-2), other gelatin-binding MMPs, an acid pH MMP and a serine protease. Specifically, we have found that increases in scleral hydrational capacity parallel increases in collagen degrading activities. Gelatin zymography reveals that eyes with 7 days of retinal image degradation have elevated levels (1.4-fold) of gelatinolytic activities at 72 and 67 kDa M(r) in equatorial and posterior pole regions of the sclera while, after 14 days of treatment, increases are no longer apparent. Lower M(r) zymographic activities at 50, 46 and 37 kDa M(r) are collectively increased in eyes treated for both 7 and 14 days (1.4- and 2.4-fold respectively) in the equator and posterior pole areas of enlarging eyes. Western blot analyses of scleral extracts with an antibody to human MMP-2 reveals immunoreactive bands at 65, 30 and 25 kDa. Zymograms incubated under slightly acidic ...Continue Reading

Citations

Jun 20, 2006·Cornea·Zygmunt MackiewiczYrjö T Konttinen
Apr 1, 2014·PloS One·Jost B JonasSonghomitra Panda-Jonas
Oct 3, 2000·Optometry and Vision Science : Official Publication of the American Academy of Optometry·N W TanS J Chew
Jul 13, 2011·European Journal of Ophthalmology·Cigdem AltanOmer Faruk Yilmaz
Dec 16, 2020·BMC Ophthalmology·Konstantin KrasseltNicole Körber
May 14, 2020·Current Eye Research·Dan-Ning HuJodi Sassoon

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