Scopoletin: Antiamyloidogenic, Anticholinesterase, and Neuroprotective Potential of a Natural Compound Present in Argyreia speciosa Roots by In Vitro and In Silico Study

Neuroscience Insights
Priya KashyapSuresh Kumar

Abstract

Alzheimer's disease (AD) is characterized by depositions of amyloid β (Aβ) peptides aggregates resulting in plaques formation in the central nervous system (CNS). This study evaluates the disease-modifying potential of scopoletin against multiple factors associated with AD such as cholinesterase enzymes, Aβ peptides, and neuroprotective properties against Aβ- and H2O2-induced cytotoxicity under in vitro conditions. Scopoletin was identified and quantified using UPLC-QTOF (ultra-high performance liquid chromatography-quadrupole time-of-flight) and high-performance liquid chromatography (HPLC), respectively. The antiamyloidogenic potential was evaluated by thioflavin T and congo red binding assay. Inhibition of key enzymes, that is, acetylcholinesterase and butyrylcholinesterase, was investigated by Ellman's assay. UPLC-QTOF analysis showed that most abundant phytoconstituent present in Argyreia speciosa hydroalcoholic root extract was scopoletin followed by festuclavine and ergometrine. Scopoletin was further quantified using novel reverse phase (RP)-HPLC method developed in this study. The neuroprotective potential of scopoletin was found to be 69% against Aβ42-induced neurotoxicity and 73% against H2O2-induced cytotoxicity in ...Continue Reading

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Citations

Aug 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Geum Seok JeongHoon Kim
Feb 27, 2021·Journal of Ethnopharmacology·Jiwei DingHua Huang
Mar 24, 2021·Neurochemistry International·Rajat SandhirNitin Kumar Singhal
Apr 14, 2021·The Journal of Pharmacy and Pharmacology·Kunnathur Murugesan SakthivelBalasubramanian Ramesh
Nov 12, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Sebastien GuesneAlice Sullivan

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