Scopoletin downregulates MMP‑1 expression in human fibroblasts via inhibition of p38 phosphorylation

International Journal of Molecular Medicine
Hae-Lim KimJoo-Won Suh

Abstract

Irradiation of keratinocytes by ultraviolet B induces cytokine production, which in turn activates fibroblasts to produce cytokines and increase matrix metallopeptidase (MMP)‑1 protein expression. The present study investigated the effect and potential mechanisms of scopoletin on the regulation of MMP‑1 expression in fibroblasts. Scopoletin was isolated from Artemisia capillaris crude extract. Treatment of fibroblasts with scopoletin resulted in a decrease in the protein expression of MMP‑1 following stimulation with human keratinocyte (HaCaT) conditioned medium. To further explore the mechanism underlying this effect, the expression levels of proteins in the mitogen‑activated protein kinase (MAPK) and nuclear factor‑κB (NF‑κB) signaling pathways were evaluated via western blot analysis. The mRNA expression levels of interleukin (IL)‑1α and tumor necrosis factor (TNF) α were evaluated via reverse transcription‑quantitative polymerase chain reaction. The effect of scopoletin on cell viability was assessed with the MTT assay. The results demonstrated that scopoletin treatment markedly decreased MMP‑1, IL‑1α and TNFα mRNA expression in fibroblasts stimulated with HaCaT conditioned medium (40 mJ/cm2), without any apparent cell cyto...Continue Reading

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Citations

May 8, 2020·Oxidative Medicine and Cellular Longevity·Emad H M HassaneinAyman M Mahmoud

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