Scopolin Prevents Adipocyte Differentiation in 3T3-L1 Preadipocytes and Weight Gain in an Ovariectomy-Induced Obese Mouse Model.

International Journal of Molecular Sciences
Eunkuk ParkSeon-Yong Jeong

Abstract

Obesity is prevalent in modern human societies. We examined the anti-obesity effects of scopolin on adipocyte differentiation in preadipocyte 3T3-L1 cells and weight loss in an ovariectomy (OVX)-induced obese mouse model. Scopolin inhibited adipocyte differentiation and lipid accumulation in the preadipocyte cells by suppressing the transcription of adipogenic-related factors, including adiponectin (Adipoq), peroxisome proliferator-activated receptor gamma (Pparg), lipoprotein lipase (Lpl), perilipin1 (Plin1), fatty acid-binding protein 4 (Fabp4), glucose transporter type 4 (Slc2a4), and CCAAT/enhancer-binding protein alpha (Cebpa). In OVX-induced obese mice, administration of scopolin promoted the reduction of body weight, total fat percentage, liver steatosis, and adipose cell size. In addition, the scopolin-treated OVX mice showed decreased serum levels of leptin and insulin. Taken together, these findings suggest that the use of scopolin prevented adipocyte differentiation and weight gain in vitro and in vivo, indicating that scopolin may be a potential bioactive compound for the treatment and prevention of obesity in humans.

References

Jun 1, 1994·Journal of Nutritional Science and Vitaminology·L J JiuI Ezawa
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·Y LeeR H Unger
Oct 1, 1993·The American Journal of Physiology·I R ReidJ Stapleton
Apr 2, 1999·Science·M F PittengerD R Marshak
Oct 5, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J WuY Ishimi
Nov 28, 2002·Journal of Lipid Research·Martin MerkelIra J Goldberg
Jan 30, 2004·Recent Progress in Hormone Research·Stephen C BenoitStephen C Woods
Apr 15, 2004·Endocrine Reviews·Robert T WatsonJeffrey E Pessin
Jun 1, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Jian WuYoshiko Ishimi
Nov 16, 2006·Phytotherapy Research : PTR·Sophie E PutnamElizabeth M Williamson
Dec 31, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Elisa FabbriniSamuel Klein
Sep 18, 2010·Trends in Endocrinology and Metabolism : TEM·Martin G MyersMichael W Schwartz
Feb 2, 2011·Archives of Gerontology and Geriatrics·Joanna DytfeldWanda Horst-Sikorska
May 6, 2011·Diabetes Care·Patricia Iozzo
May 20, 2011·The Veterinary Journal·Rebecca Ricci, Francesca Bevilacqua
Feb 14, 2012·Current Opinion in Endocrinology, Diabetes, and Obesity·Olga T HardySilvia Corvera
Jan 1, 2012·Adipocyte·Christopher ChurchMatthew Rodeheffer
Feb 26, 2014·Critical Reviews in Food Science and Nutrition·Shuna ZhaoSang-Moo Kim
Nov 29, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eunkuk ParkSeon-Yong Jeong
Jan 23, 2016·International Journal of Molecular Sciences·Dorothy MosetiWoo-Kyun Kim
Feb 6, 2016·International Journal of Molecular Medicine·Jae-Hyun KimYoungjoo Sohn
Oct 6, 2016·Experimental and Therapeutic Medicine·Shu-Zhong JiangYi Liu
Dec 10, 2016·Journal of Agricultural and Food Chemistry·Chuhan FuZhengquan Su
May 26, 2018·International Journal of Molecular Sciences·Nicholas Ekow ThomfordKevin Dzobo
Jul 26, 2018·The Journal of Biological Chemistry·Jee Hyung SohnJae Bum Kim
Jan 30, 2019·Frontiers in Neuroscience·Leticia Forny-GermanoMarcelo Nunes do Nascimento Vieira
Sep 22, 2019·Nature Reviews. Endocrinology·Shimona Starling
Apr 26, 2020·International Journal of Pharmaceutics·Amrita BanerjeeJagdish Singh

❮ Previous
Next ❯

Methods Mentioned

BETA
reverse-transcription PCR
enzyme-linked immunosorbent assay
Assay

Software Mentioned

CaseViewer
SPSS
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.