Screening actinomycetes for extracellular peroxidase activity.

Applied and Environmental Microbiology
D K MercerA J McCarthy

Abstract

A diverse collection of actinomycete strains were screened for production of extracellular peroxidase activity by adapting a chemiluminescence analysis system developed for horseradish peroxidase-based enzyme-linked immunosorbent assay. Extracellular peroxidase activity was found to be common but quantitatively variable, and this rapid and sensitive screening system permitted identification of a small group of high-producing strains. A range of spectrophotometric assays were compared for the measurement of peroxidase activity in concentrated culture supernatants of two selected thermophilic streptomycetes. Of these, the peroxide-dependent oxidation of 2,4-dichlorophenol was identified as the most robust and reproducible assay for quantitative studies.

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Citations

Nov 7, 2003·Applied and Environmental Microbiology·Ulrike BurkertJakob Pernthaler
Nov 2, 2013·Journal of Applied Microbiology·A MusengiS G Burton
Jun 26, 2020·Frontiers in Microbiology·Carolyne Caetano GonçalvesNádia Skorupa Parachin
Jul 2, 2011·Antonie van Leeuwenhoek·Marilize Le Roes-HillStephanie Burton
Jul 12, 2012·Applied Microbiology and Biotechnology·Christoph A GasserPhilippe F-X Corvini
Sep 26, 2001·Applied and Environmental Microbiology·M G MasonM T Wilson
Feb 1, 2011·Applied Biochemistry and Biotechnology·Marilize le Roes-HillStephanie Gail Burton
Oct 12, 2018·Biotechnology and Applied Biochemistry·Ayodeji O FaladeUchechukwu U Nwodo
Mar 21, 2008·Environmental Science & Technology·Heidi M Bialk, Joel A Pedersen
Oct 12, 2000·Enzyme and Microbial Technology·M GablerL Fischer

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