Screening and Antifungal Activity of a β -Carboline Derivative against Cryptococcus neoformans and C. gattii

International Journal of Microbiology
Kátia Santana CruzJoão Vicente Braga de Souza

Abstract

Cryptococcosis is a fungal disease of bad prognosis due to its pathogenicity and the toxicity of the drugs used for its treatment. The aim of this study was to investigate the medicinal potential of carbazole and β-carboline alkaloids and derivatives against Cryptococcus neoformans and C. gattii. MICs were established in accordance with the recommendations of the Clinical and Laboratory Standards Institute for alkaloids and derivatives against C. neoformans and C. gattii genotypes VNI and VGI, respectively. A single active compound was further evaluated against C. neoformans genotypes VNII, VNIII, and VNIV, C. gattii genotypes VGI, VGIII, and VGIV, Candida albicans ATCC 36232, for cytotoxicity against the MRC-5 lineage of human fibroblasts and for effects on fungal cells (cell wall, ergosterol, and leakage of nucleic acids). Screening of 11 compounds revealed 8-nitroharmane as a significant inhibitor (MIC 40 μg/mL) of several C. neoformans and C. gattii genotypes. It was not toxic to fibroblasts (IC50 > 50 µg/mL) nor did it alter fungal cell walls or the concentration of ergosterol in C. albicans or C. neoformans. It increased leakage of substances that absorb at 260 nm. The synthetic β-carboline 8-nitroharmane significantly in...Continue Reading

References

Jan 1, 1992·Journal of Ethnopharmacology·A AhmadS Siddiqui
May 26, 1997·Journal of Immunological Methods·G R NakayamaZ Parandoosh
Jun 20, 2000·Antimicrobial Agents and Chemotherapy·C S Lunde, I Kubo
Dec 29, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·T HerraizH Guillén
Jan 6, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·John R PerfectTania C Sorrell
Dec 22, 2011·International Journal of Microbiology·Patrick VandeputteAlix T Coste
Jan 21, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Héctor CarrascoSusana Zacchino
Jul 8, 2014·Annals of Clinical Microbiology and Antimicrobials·Maria do Perpetuo Socorro Borges Carriço FerreiraJoão Vicente Braga Souza
May 20, 2015·Fungal Genetics and Biology : FG & B·Alexander Idnurm, Xiaorong Lin
Nov 28, 2016·Food Microbiology·Gabriela M OlmedoSabrina I Volentini
May 10, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Claudia ZaniAnnamaria Buschini
May 13, 2017·Nature Reviews. Drug Discovery·John R Perfect
May 19, 2017·Nature Communications·Flore MiettonCarlo Petosa
Jul 14, 2017·Nanomedicine·Katarzyna NiemirowiczRobert Bucki
Oct 25, 2017·Scientific Reports·Lorenzo GuzzettiMassimo Labra
Nov 14, 2017·Current Clinical Microbiology Reports·Jane A O'HalloranAndrej Spec

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.