Screening and application of a broad-spectrum aptamer for acyclic guanosine analogues.

Analytical and Bioanalytical Chemistry
Le RenZhouping Wang

Abstract

Acyclic guanosine analogues, a class of widely used antiviral drugs, can cause chronic toxicity and virus resistance. Therefore, it is essential to establish rapid and accurate methods to detect acyclic guanosine analogues. In this study, five acyclic guanosine analogues (acyclovir, famciclovir, ganciclovir, penciclovir, and valaciclovir) were used as positive targets to obtain broad-spectrum aptamers through Capture-SELEX technology. Real-time quantitative PCR (Q-PCR) was used to monitor the aptamer SELEX process. After the sixteen rounds of selection against mixed targets, sequences were obtained by high-throughput sequencing (HTS). Furthermore, a broad-spectrum aptamer, named CIV6, was found as the higher performance aptamer that was suitable for five acyclic guanosine analogues by graphene oxide (GO) polarization and fluorescence assay. Finally, the aptamer CIV6 was used to construct GO fluorescence assay to detect five acyclic guanosine analogues. The limits of detection (LOD) of acyclovir, famciclovir, ganciclovir, penciclovir, and valaciclovir were 0.48 ng·mL-1, 0.53 ng·mL-1, 0.50 ng·mL-1, 0.56 ng·mL-1, and 0.38 ng·mL-1, respectively.

References

Aug 30, 1990·Nature·A D Ellington, J W Szostak
Dec 12, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·R WhiteB Sullenger
Jan 31, 2002·Journal of Chromatography. B, Biomedical Sciences and Applications·A LoregianE F De Palo
May 15, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hung C VoStephen L Sacks
Sep 2, 2009·Journal of the American Veterinary Medical Association·Jennifer L DavisJim E Riviere
Jan 21, 2012·Biotechnology Letters·Li WangJian Tu
Jan 18, 2015·Applied Biochemistry and Biotechnology·Neelam Verma, Atul Bhardwaj
Nov 9, 2015·Biosensors & Bioelectronics·C ReinemannB Strehlitz
May 14, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Pengqian MuJing Qiu
Jun 27, 2018·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jian ShiHao-Jie Zhu
Mar 31, 2019·Nucleic Acids Research·Weijuan YangYi Xiao
Apr 28, 2019·Biochemical and Biophysical Research Communications·Ligang XingJidong Yang
May 1, 2019·Talanta·Jianhua YanZhenbao Liu
Jul 10, 2019·ACS Applied Materials & Interfaces·Magdalena PachotaMaria Nowakowska
Aug 27, 2019·Analytica Chimica Acta·Huili TianZhouping Wang
Oct 22, 2019·European Journal of Pharmacology·Gabriela Pastuch-GawołekIlona Wandzik
Jun 6, 2020·Analytical and Bioanalytical Chemistry·Raja ChinnappanMohammed Zourob
Jul 29, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anna A MaslovaMikhail S Novikov
Aug 12, 2020·Analytical and Bioanalytical Chemistry·Shiwei LiKenneth McNatty

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

© 2021 Meta ULC. All rights reserved