Screening and confirmation methods for GHB determination in biological fluids

Analytical and Bioanalytical Chemistry
Ann-Sofie M E IngelsChristophe P Stove

Abstract

The purpose of this review is to provide a comprehensive overview of reported methods for screening and confirmation of the low-molecular-weight compound and drug of abuse gamma-hydroxybutyric acid (GHB) in biological fluids. The polarity of the compound, its endogenous presence, its rapid metabolism after ingestion, and its instability during storage (de novo formation and interconversion between GHB and its lactone form gamma-butyrolactone) are challenges for the analyst and for interpretation of a positive result. First, possible screening procedures for GHB are discussed, including colorimetric, enzymatic, and chromatography-based procedures. Confirmation methods for clinical and forensic cases mostly involve gas chromatography (coupled to mass spectrometry), although liquid chromatography and capillary zone electrophoresis have also been used. Before injection, sample-preparation techniques include (a combination of) liquid-liquid, solid-phase, or headspace extraction, and chemical modification of the polar compound. Also simple "dilute-and-shoot" may be sufficient for urine or serum. Advantages, limitations, and trends are discussed.

References

Feb 11, 1971·Biochimica Et Biophysica Acta·J C Brown, P Doty
Aug 1, 1981·The International Journal of the Addictions·M J HoesP J Guelen
Jan 1, 1993·European Journal of Clinical Pharmacology·P PalatiniS D Ferrara
Jun 1, 1993·Journal of Pharmaceutical and Biomedical Analysis·S D FerraraP Palatini
Jan 1, 1997·Journal of Toxicology. Clinical Toxicology·H K LouagiePaul A Calle
Jun 13, 1998·Annals of Emergency Medicine·J LiA Woeckener
Jun 12, 1999·Therapeutic Drug Monitoring·N R Badcock, R Zotti
Jun 16, 1999·Journal of Analytical Toxicology·Mahmoud A Elsohly, S J Salamone
Sep 17, 1999·Journal of Analytical Toxicology·R R McCuskerB A Goldberger
Mar 22, 2000·Forensic Science International : Synergy·Albert A Elian
Jun 23, 2000·The Journal of Emergency Medicine·M IngelsR F Clark
Feb 15, 2001·Annals of Emergency Medicine·Jo Ellen DyerB A Hyma
Mar 20, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·C A de VriendtFrans M Belpaire
May 30, 2001·Forensic Science International : Synergy·Marc A LeBeauB Levine
Aug 14, 2001·Journal of Analytical Toxicology·Y M PanH H McCurdy
Oct 3, 2001·The American Journal on Addictions·K MiottoR Rawson
Oct 6, 2001·Forensic Science International : Synergy·Albert A Elian
Mar 13, 2002·Toxicology Letters·Koichi SakuradaKen-ichi Yoshida
May 9, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Benoît BlanchetAlain Astier
Jun 18, 2002·American Journal of Health-system Pharmacy : AJHP : Official Journal of the American Society of Health-System Pharmacists·Kelly M SmithFrank Romanelli
Jun 27, 2002·Forensic Science International : Synergy·William C Alston, Karno Ng
Jul 20, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Toshihiro ShinkaTomiko Kuhara
Aug 15, 2002·Forensic Science International : Synergy·Albert A Elian
Sep 11, 2002·Journal of Analytical Toxicology·Marc A LeBeauMarilyn A Huestis
Dec 28, 2002·Journal of Analytical Toxicology·Sarah Kerrigan
Jan 22, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yousuke FukuiKazumasa Yamashita
Jan 30, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Cara PolsonRussell Grant
May 14, 2003·Forensic Science International : Synergy·Simon Elliott
May 14, 2003·Forensic Science International : Synergy·Ingrid J Bosman, Klaas J Lusthof
Jun 6, 2003·Forensic Science International : Synergy·C Lora-TamayoE Molina
Jun 28, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Marion VillainPascal Kintz
Jun 28, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Masahiko KimuraSeiji Yamaguchi
Jul 25, 2003·Journal of Chromatography. a·Roger M Smith
Dec 31, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Federica BortolottiFranco Tagliaro
Mar 19, 2004·Journal of Forensic Sciences·Dawn T BravoStanley M Parsons
Mar 26, 2004·Forensic Science International : Synergy·Simon ElliottAmanda Symonds
Jun 19, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ho-Leung FungSun-Mi Fung
Jul 9, 2004·Forensic Science International : Synergy·Pascal KintzBertrand Ludes
Jul 17, 2004·Therapeutic Drug Monitoring·Simon P Elliott
Aug 10, 2004·Toxicological Reviews·Cynthia Morris-Kukoski
Nov 13, 2004·Journal of Analytical Toxicology·Rudolf BrenneisenDavid E Watson
Nov 13, 2004·Journal of Analytical Toxicology·C E CrookesJ H Galloway
Apr 6, 2005·Journal of Analytical Toxicology·Laureen J MarinettiSawait Kanluen
Aug 18, 2005·Journal of Analytical Toxicology·Susan Mazarr-Proo, Sarah Kerrigan
Sep 20, 2005·Journal of Analytical Toxicology·Pascal KintzGeorges Leonetti
Jan 13, 2006·Therapeutic Drug Monitoring·Jan F Van Bocxlaer
Jan 25, 2006·Annals of Emergency Medicine·Ilene B AndersonPaul D Blanc
Jul 29, 2006·Journal of Analytical Toxicology·Richard PaulAlan Guwy
Aug 16, 2006·Forensic Science International : Synergy·Eckhard Kaufmann, Andreas Alt
Oct 13, 2006·Forensic Science International : Synergy·Marc A LeBeauAnna Deakin
Nov 16, 2006·Annals of the New York Academy of Sciences·Sergio AbanadesRafael de la Torre
Dec 13, 2006·Forensic Science International : Synergy·Stacy D BrownBoyd J Pritchard
Mar 17, 2007·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Uttam GargGary Wasserman
May 1, 2007·Analytical and Bioanalytical Chemistry·Thomas Kraemer, Liane D Paul
May 16, 2007·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Diederik K Van SassenbroeckWalter A Buylaert
Jul 31, 2007·Forensic Science International : Synergy·Aino KankaanpääKari Ariniemi
Dec 21, 2007·Journal of Analytical Toxicology·Alan Wayne JonesFredrik C Kugelberg
Apr 24, 2008·Journal of Analytical Toxicology·Giorgia De Paoli, Suzanne Bell
Dec 3, 2008·International Journal of Legal Medicine·Fredrik C KugelbergAlan Wayne Jones
Apr 27, 2010·Forensic Science International : Synergy·H AndresenS Iwersen-Bergmann
Sep 10, 2010·The American Journal of Emergency Medicine·Deborah L ZvosecJo Ellen Dyer
Sep 14, 2010·Analytical and Bioanalytical Chemistry·Ann-Sofie M E IngelsChristophe P Stove
Mar 29, 2011·Journal of Analytical Toxicology·Giorgia De PaoliDerrick J Pounder
Nov 8, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Albert A Elian, Jeffery Hackett
Nov 23, 2011·Therapeutic Drug Monitoring·Lara HasanAndré Scholer
Jan 10, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sandra Rinne DahlDag Helge Strand
Feb 22, 2012·Neuroscience and Biobehavioral Reviews·Jan G C van AmsterdamRaymond J M Niesink
Jun 23, 2012·Journal of Analytical Toxicology·Vincent GrenierPascal Mireault
Jul 4, 2012·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Leo J SchepBruno Mégarbane
Sep 29, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Robert NeijzenErik van Maarseveen
Mar 15, 2013·Journal of Analytical Toxicology·H Andresen-StreichertS Iwersen-Bergmann
Apr 25, 2013·Journal of Analytical Toxicology·Ida Nymann PetersenTorben Breindahl
May 29, 2013·Analytical and Bioanalytical Chemistry·Marek DziadoszJörg Teske
Jul 23, 2013·Molecular Genetics and Metabolism·Christophe P StoveWilly E Lambert

Citations

Jul 27, 2015·International Journal of Legal Medicine·Stephanie HanischGisela Skopp
Feb 24, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Marek Dziadosz
Oct 12, 2018·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·F P Busardò, Alan Wayne Jones
Oct 12, 2018·Sudebno-meditsinskaia ekspertiza·V D IsakovN V Saigushkin
Sep 11, 2014·Annals of Clinical Biochemistry·Luc HeytensCleo L Crunelle
Nov 28, 2020·Journal of Analytical Toxicology·Sarah M R Wille, Simon P Elliott

Related Concepts

4-hydroxybutyric acid
Body Fluids
Calorimetry
Chromatography, Gas-Liquid
Liquid Chromatography
Filtration
Hydroxybutyric Acids
Chromatography, Gas-Liquid-Mass Spectrometry
Mass Spectrometry
4-Butyrolactone

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