Screening and construction of Saccharomyces cerevisiae strains with improved multi-tolerance and bioethanol fermentation performance

Bioresource Technology
Dao-Qiong ZhengYuhua Zhao

Abstract

In this study, a systemic analysis was initially performed to investigate the relationship between fermentation-related stress tolerances and ethanol yield. Based on the results obtained, two elite Saccharomyces cerevisiae strains, Z8 and Z15, with variant phenotypes were chosen to construct strains with improved multi-stress tolerance by genome shuffling in combination with optimized initial selection. After three rounds of genome shuffling, a shuffled strain, YZ1, which surpasses its parent strains in osmotic, heat, and acid tolerances, was obtained. Ethanol yields of YZ1 were 3.11%, 10.31%, and 10.55% higher than those of its parent strains under regular, increased heat, and high gravity fermentation conditions, respectively. YZ1 was applied to bioethanol production at an industrial scale. Results demonstrated that the variant phenotypes from available yeast strains could be used as parent stock for yeast breeding and that the genome shuffling approach is sufficiently powerful in combining suitable phenotypes in a single strain.

References

Nov 1, 1987·Molecular and Cellular Biology·J H McCuskerJ E Haber
Jan 1, 1997·Journal of Industrial Microbiology & Biotechnology·J G LewisK Watson
May 29, 1999·The Journal of Biological Chemistry·J LeeM B Toledano
Jan 12, 2001·FEMS Microbiology Reviews·J François, J L Parrou
Feb 17, 2001·Molecular Biology of the Cell·H C CaustonR A Young
Feb 8, 2002·Nature·Ying-Xin ZhangStephen B del Cardayré
Mar 23, 2005·FEMS Yeast Research·Z Petek CakarUwe Sauer
Jan 18, 2006·Applied Microbiology and Biotechnology·Shu-Jing SunZhi-Bin Lin
Dec 26, 2007·Bioresource Technology·Oscar J Sánchez, Carlos A Cardona
Oct 11, 2008·Journal of Industrial Microbiology & Biotechnology·Dong-jian ShiKui-ming Wang
Jan 7, 2009·Bioprocess and Biosystems Engineering·Thai Nho DinhHiroshi Shimizu
Jan 21, 2009·Biotechnology Letters·Lihua Hou
Apr 7, 2009·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wei PengJunchuan Qin
May 26, 2009·Biotechnology Advances·Jixian GongXueming Zhao
Oct 27, 2009·Applied and Environmental Microbiology·Burkhard OtteStefan Jennewein

Citations

Dec 19, 2013·Applied Microbiology and Biotechnology·Dao-Qiong ZhengXue-Chang Wu
Mar 7, 2014·Applied Microbiology and Biotechnology·Damien Biot-Pelletier, Vincent J J Martin
Dec 20, 2015·Journal of Bioscience and Bioengineering·Liman GaoGuiyan Liu
Mar 8, 2011·Journal of Evolutionary Biology·R DharA Wagner
Apr 15, 2014·FEMS Microbiology Reviews·Jan SteenselsKevin J Verstrepen
Jun 26, 2012·Journal of Biotechnology·Akihiko KondoFumio Matsuda
May 18, 2016·Applied Microbiology and Biotechnology·Kristoffer KrogerusBrian Gibson
Mar 20, 2014·Biotechnology Journal·Esther Gamero-SandemetrioEmilia Matallana
Jun 7, 2017·FEMS Yeast Research·Quinten DeparisJohan M Thevelein
Jan 13, 2018·Frontiers in Bioengineering and Biotechnology·Kentaro InokumaAkihiko Kondo
Jan 16, 2018·Applied Microbiology and Biotechnology·Ke ZhangDao-Qiong Zheng
Oct 16, 2018·Food Science and Biotechnology·Monika Kordowska-WiaterKrzysztof Kostro
Apr 22, 2017·Applied Microbiology and Biotechnology·Ke ZhangXue-Chang Wu

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