Screening and determination for potential acetylcholinesterase inhibitory constituents from ginseng stem-leaf saponins using ultrafiltration (UF)-LC-ESI-MS2

Phytochemical Analysis : PCA
Yinping YangJiaming Sun

Abstract

Previous studies have demonstrated that several ginsenosides have remarkable inhibitory effect on acetylcholinesterase (AChE). In the present study, ginseng stem-leaf saponins (GSLS) can improve learning and memory of Alzheimer's disease patients. However, much comprehensive information regarding AChE inhibition of GSLS and its metabolites is yet unknown. The present study aims to screen and determine the potential of AChE inhibitors (AChEIs) from GSLS. The active fraction of the GSLS detected in vitro AChE inhibition assays was selected as a starting material for the screening of the potential of AChEIs using ultrafiltration liquid chromatography coupled to electrospray ionisation tandem mass spectrometry (UF-LC-ESI-MS2 ). The results showed that 31 ginsenosides were identified with analysis using rapid resolution liquid chromatography with a diode array detector combined with electrospray ionisation tandem mass spectrometry (RRLC-DAD-ESI-MS2 ) from the active fraction, and there are 27 compounds with AChE binding activity. Among them, 11 ginsenosides were evaluated and confirmed using in vitro enzymatic assay, and ginsenosides F1 , Rd, Rk3 , 20(S)-Rg3 , F2 and Rb2 were found to possess strong AChE inhibitory activities. The p...Continue Reading

References

Mar 14, 2000·International Journal of Geriatric Psychiatry·G Livingston, C Katona
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE
Aug 5, 2009·Biological & Pharmaceutical Bulletin·Hyun Ah JungJae Sue Choi
Jul 23, 2015·Journal of Ginseng Research·Yeonju Lee, Seikwan Oh
Jun 9, 2016·Journal of Ethnopharmacology·Ran Joo ChoiHyun Ah Jung
Oct 25, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jinbin YuanChun-Su Yuan
Jul 18, 2017·Journal of Molecular Graphics & Modelling·M Rejwan AliMihaly Mezei
Jul 25, 2017·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sainan LiYuchi Zhang

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Citations

Dec 5, 2019·Current Topics in Medicinal Chemistry·Jiayang XieBing Niu
Jun 6, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiao-Chen GaoJia-Ming Sun

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