Screening and development of enzymes for determination and transformation of amino acids

Bioscience, Biotechnology, and Biochemistry
Yasuhisa Asano


The high stereo- and substrate specificities of enzymes have been utilized for micro-determination of amino acids. Here, I review the discovery of l-Phe dehydrogenase and its practical use in the diagnosis of phenylketonuria in more than 5,400,000 neonates over two decades in Japan. Screening and uses of other selective enzymes for micro-determination of amino acids have also been discussed. In addition, novel enzymatic assays with the systematic use of known enzymes, including assays based on a pyrophosphate detection system using pyrophosphate dikinase for a variety of l-amino acids with amino-acyl-tRNA synthetase have been reviewed. Finally, I review the substrate specificities of a few amino acid-metabolizing enzymes that have been altered, using protein engineering techniques, mainly for production of useful chemicals, thus enabling the wider use of natural enzymes.


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Mar 28, 1998·Archives of Microbiology·Yasuhisa Asano, M Tanetani
Jan 25, 2006·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nobutoshi KibaKoki Yokotsuka
Oct 19, 2006·Analytical Biochemistry·Shinjiro TachibanaYasuhisa Asano
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Oct 16, 2009·Applied Microbiology and Biotechnology·Sergio Martínez-RodríguezFrancisco Javier Las Heras-Vázquez
Nov 16, 2010·Analytical Biochemistry·Techawaree Ueatrongchit, Yasuhisa Asano
May 19, 2012·Analytical Biochemistry·Motoki Matsuda, Yasuhisa Asano
Jun 19, 2012·Journal of Bioscience and Bioengineering·Kimiyasu IsobeYasuhisa Asano
Nov 19, 2013·Analytical Biochemistry·Masafumi KameyaYasuhisa Asano
Mar 19, 2014·Enzyme and Microbial Technology·Masafumi Kameya, Yasuhisa Asano
Sep 2, 2014·Molecular Genetics and Metabolism·Urh GroseljTadej Battelino
Apr 22, 2015·Bioscience, Biotechnology, and Biochemistry·Daisuke Matsui, Yasuhisa Asano
Jul 29, 2016·The Journal of Biological Chemistry·Janis J FüllerJürgen Moser
Sep 21, 2016·The Journal of Physical Chemistry. B·Shogo NakanoYasuhisa Asano
Dec 13, 2016·Applied Microbiology and Biotechnology·Masafumi Kameya, Yasuhisa Asano
Jan 1, 2016·The European Physical Journal. C, Particles and Fields·Atlas CollaborationL Zwalinski

Related Concepts

Metabolic Biotransformation
Neonatal Screening
Amino Acyl-tRNA Synthetases
Protein Engineering
Microbiology Procedure
Determination Aspects
Natural Products
Screening Generic

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