Screening and functional identification of lncRNAs in antler mesenchymal and cartilage tissues using high-throughput sequencing.

Scientific Reports
Dan-Yang ChenWei Hu

Abstract

Long non-coding RNA (lncRNA) is a transcription product of the mammalian genome that regulates the development and growth in the body. The present study aimed to analyze the expression dynamics of lncRNA in sika antler mesenchymal and cartilage tissues by high-throughput sequencing. Bioinformatics was applied to predict differentially expressed lncRNAs and target genes and screen lncRNAs and mRNAs related to osteogenic differentiation, cell proliferation, and migration. Finally, the expression of the lncRNAs and target genes were analyzed by qRT-PCR. The results showed that compared to the cartilage tissue, the transcription levels of lncRNA and mRNA, 1212 lncRNAs and 518 mRNAs, in mesenchymal tissue were altered significantly. Thus, a complex interaction network was constructed, and the lncRNA-mRNA interaction network correlation related to osteogenic differentiation, cell proliferation, and migration was analyzed. Among these, the 26 lncRNAs and potential target genes were verified by qRT-PCR, and the results of qRT-PCR were consistent with high-throughput sequencing results. These data indicated that lncRNA promotes the differentiation of deer antler mesenchymal tissue into cartilage tissue by regulating the related osteogen...Continue Reading

References

Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Sep 20, 2001·Current Pharmaceutical Design·A A Adjei
Aug 6, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J Price, S Allen
Jul 18, 2006·Nucleic Acids Research·Jianmin WuLiping Wei
May 5, 2009·RNA Biology·Riki KurokawaChristopher K Glass
Jul 16, 2010·Journal of Neuroscience Research·Wolfgang Pita-ThomasManuel Nieto-Diaz
Oct 29, 2010·Genome Biology·Simon Anders, Wolfgang Huber
Jan 13, 2011·International Journal of Cancer. Journal International Du Cancer·Riina KuuseloAnne Kallioniemi
May 20, 2011·Nucleic Acids Research·Robert D FinnSean R Eddy
Dec 29, 2011·Frontiers in Bioscience (Landmark Edition)·Manuel Nieto-DiazRodrigo Martinez-Maza
Dec 4, 2013·Nature Reviews. Genetics·Alessandro Fatica, Irene Bozzoni
Jan 29, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Kazuhiko MatsuokaSunao Takeshita
Jul 22, 2014·The International Journal of Biochemistry & Cell Biology·Chunyi LiChris McMahon
Dec 24, 2014·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Yaghoub SafdariSafar Farajnia
Feb 19, 2015·Nature Biotechnology·Mihaela PerteaSteven L Salzberg
Mar 10, 2015·Nature Methods·Daehwan KimSteven L Salzberg
Oct 18, 2015·Nucleic Acids Research·Minoru KanehisaMao Tanabe
Oct 26, 2016·JCI Insight·Toshifumi FujiwaraHaibo Zhao
Jul 14, 2017·The American Journal of Pathology·Jin-Sil ParkMi-La Cho
Jan 19, 2018·Oncology Letters·Zhijun LiaoDexin Lin
Mar 13, 2018·Circulation Journal : Official Journal of the Japanese Circulation Society·Koh OnoTakeshi Kimura
Jul 17, 2018·Experimental Cell Research·Taomei YangGuangwei Wei
Oct 16, 2018·Nucleic Acids Research·Minoru KanehisaMao Tanabe
Oct 24, 2018·Frontiers in Endocrinology·Qian-Yuan WuFeng Lai Yuan
May 3, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Cheng JuZhiping Zhang
Aug 24, 2019·Protein Science : a Publication of the Protein Society·Minoru Kanehisa
Oct 18, 2019·Frontiers in Oncology·Suraksha TamangAklank Jain

❮ Previous
Next ❯

Citations

Mar 27, 2021·Journal of Proteome Research·Zhen Dong, Dawn Coates

❮ Previous
Next ❯

Methods Mentioned

BETA
gene knockdown
RNA-seq
electrophoresis

Software Mentioned

Cytoscape
SPSS
Stringtie
HMMER
R
FastQ
CNCI
CPC
HISAT2
MCL

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.