Screening and functional prediction of differentially expressed circRNAs in proliferative human aortic smooth muscle cells.

Journal of Cellular and Molecular Medicine
Wei ChenShao-Guang Sun

Abstract

Vascular smooth muscle cell (VSMC) proliferation is the pathological base of vascular remodelling diseases. Circular RNAs (circRNAs) are important regulators involved in various biological processes. However, the function of circRNAs in VSMC proliferation regulation remains largely unknown. This study was conducted to identify the key differentially expressed circRNAs (DEcircRNAs) and predict their functions in human aortic smooth muscle cell (HASMC) proliferation. To achieve this, DEcircRNAs between proliferative and quiescent HASMCs were detected using a microarray, followed by quantitative real-time RT-PCR validation. A DEcircRNA-miRNA-DEmRNA network was constructed, and functional annotation was performed using Gene Ontology (GO) and KEGG pathway analysis. The function of hsa_circ_0002579 in HASMC proliferation was analysed by Western blot. The functional annotation of the DEcircRNA-miRNA-DEmRNA network indicated that the four DEcircRNAs might play roles in the TGF-β receptor signalling pathway, Ras signalling pathway, AMPK signalling pathway and Wnt signalling pathway. Twenty-seven DEcircRNAs with coding potential were screened. Hsa_circ_0002579 might be a pro-proliferation factor of HASMC. Overall, our study identified th...Continue Reading

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Methods Mentioned

BETA
Feature Extraction
PCR
electrophoresis
Transfection

Software Mentioned

GeneCodis
GeneSpring GX
Cytoscape
MiRanda
Agilent Feature Extraction
TargetScan
Agilent GeneSpring GX
Arraystar
DEcircRNA

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