Screening and identification of a six-cytokine biosignature for detecting TB infection and discriminating active from latent TB

Journal of Translational Medicine
Sen WangWenhong Zhang

Abstract

The early and accurate diagnosis of tuberculosis (TB) is critical for controlling the global TB epidemic. Although early studies have supported the potential role of cytokine biomarkers in blood for the diagnosis of TB, this method requires further investigation and validation in different populations. A set of biomarkers that can discriminate between active TB (ATB) and latent TB infection (LTBI) remains elusive. In the current study, we organized two retrospective cohorts and one prospective cohort to investigate the immune responses at different clinical stages of TB infection, as determined by candidate cytokine biomarkers detected with a multiplex cytokine platform. Using a pre-established diagnostic algorithm, participants were classified as ATB, LTBI, and TB uninfected controls (CON). Based on our multiplex cytokine assay, a multi-cytokine biosignature was modelled for the optimal recognition of the different TB infection status. Our analysis identified a six-cytokine biosignature of TB-antigen stimulated IFN-γ, IP-10, and IL-1Ra, and unstimulated IP-10, VEGF, and IL-12 (p70) for a biomarker screening group (n = 88). The diagnostic performance of the biosignature was then validated using a biomarker validation cohort (n ...Continue Reading

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Citations

Feb 9, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Paulo RanaivomananaVoahangy Rasolofo
Mar 19, 2021·Frontiers in Immunology·Bih H ChendiAnne M Dyrhol-Riise
May 1, 2021·Pathogens·Magdalena DruszczynskaWieslawa Rudnicka
Nov 24, 2021·MBio·Diogo SilvérioMargarida Saraiva

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

bio
QFT
GraphPad Prism
R
plex manager
SVM

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