Screening and identification of differentially expressed microRNAs in diffuse large B-cell lymphoma based on microRNA microarray.

Oncology Letters
Hai-Xia GaoXin-Xia Li

Abstract

Diffuse large B-cell lymphoma (DLBCL) is the most common type of B-cell non-Hodgkin lymphoma in adults and the pathogenesis of DLBCL is multifactorial and complex. Understanding the molecular mechanisms involved in DLBCL is important to identify new therapeutic targets. The present study aimed to screen and identify differentially expressed microRNAs (miRNAs/miRs) between diffuse large B-cell lymphoma (DLBCL) and control [lymph node reactive hyperplasia (LRH)] groups, and to investigate whether miRNAs associated with DLBCL could serve as potential therapeutic targets. In total, 5 DLBCL experimental samples and 5 control samples were obtained from fresh patient tissues. Firstly, the fresh samples were analyzed using miRNA microarray to identify differentially expressed miRNAs. Next, three databases (TargetScan, microRNA.org and PITA) were used to predict by intersection the potential target genes of the 204 differential miRNAs identified, and a Venn diagram of the results was performed. Subsequently, the target genes of differential miRNAs were analyzed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis. Finally, to validate the miRNA microarray data, reverse transcription-quantitative PCR (RT-qPCR) wa...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 13, 2013·Nature Reviews. Clinical Oncology·Mark RoschewskiWyndham H Wilson
Dec 4, 2016·Hematology·Bertrand Coiffier, Clémentine Sarkozy
Nov 24, 2017·Pathology·Shaoying LiL Jeffrey Medeiros
Nov 30, 2018·Molecular Therapy. Nucleic Acids·Arulraj NadaradjanePierre-François Cartron
Feb 20, 2019·Journal of Cellular Physiology·Monireh KhordadmehrBehzad Baradaran
Apr 11, 2019·American Journal of Physiology. Cell Physiology·Zhiwen LiuZheng Dong
Jun 20, 2019·Cancer Management and Research·Zhi-Min ChenDan-Ming Wei
Nov 22, 2019·Scientific Reports·Afshin BeheshtiAndrew M Evens
Jan 11, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eui-Jung ParkTae-Hwan Kwon
Mar 25, 2020·Genes & Genomics·Hyein ShimSang-Woo Kim
May 6, 2020·Journal of Cancer·Vladimir A ValeraMaria J Merino
May 29, 2020·Nature·Lorenzo BarontiKatja Petzold

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