Screening and identification of MicroRNAs expressed in perirenal adipose tissue during rabbit growth

Lipids in Health and Disease
Guoze WangSongjia Lai

Abstract

MicroRNAs (miRNAs) regulate adipose tissue development, which are closely related to subcutaneous and intramuscular fat deposition and adipocyte differentiation. As an important economic and agricultural animal, rabbits have low adipose tissue deposition and are an ideal model to study adipose regulation. However, the miRNAs related to fat deposition during the growth and development of rabbits are poorly defined. In this study, miRNA-sequencing and bioinformatics analyses were used to profile the miRNAs in rabbit perirenal adipose tissue at 35, 85 and 120 days post-birth. Differentially expressed (DE) miRNAs between different stages were identified by DEseq in R. Target genes of DE miRNAs were predicted by TargetScan and miRanda. To explore the functions of identified miRNAs, Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed. Approximately 1.6 GB of data was obtained by miRNA-seq. A total of 987 miRNAs (780 known and 207 newly predicted) and 174 DE miRNAs were identified. The miRNAs ranged from 18 nt to 26 nt. GO enrichment and KEGG pathway analyses revealed that the target genes of the DE miRNAs were mainly involved in zinc ion binding, regulation of cell growth, ...Continue Reading

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Citations

Dec 2, 2020·Animals : an Open Access Journal From MDPI·Jiahao ShaoSongjia Lai

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Methods Mentioned

BETA
RNA Assay
PCR
miRNA-seq
ubiquitination

Software Mentioned

FastQC
SPSS Statistics
Primer
R
miR
DEGseq
- BLAST
DEGseq R
miRanda
TargetScan

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