Screening and identification of receptor antagonist for shiga toxin from random peptides displayed on filamentous bacteriophages

Science in China. Series C, Life Sciences
Z HanC Huang

Abstract

The bacteriophage clones which can bind with shiga toxin B subunit (StxB) and inhibit cytotoxicity of shiga toxin were obtained by using antibody capturing method from a 15-mer random peptide library displayed on the surface of bacteriophage fd. Among them, one peptide encoded by the random DNA region of a selected bacteriophage (A12) was synthesized and tested in vitro and in vim, where the peptide competed with the receptor of shiga toxin to bind StxB, and inhibited the cytotoxicity and enterotoxicity of shiga toxin. The peptide can also block other apparently unrelated StxB binding bacteriophage (A3), which suggests that there are overlapping StxB interaction sites for those ligands with different sequences. The results provide a demonstration of bacteriophage display to screen peptide ligands for a small and/or unable biotinylated molecule by antibodies-capturing strategy, and take the lead for the development of receptor antagonists for shiga toxin.

References

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Citations

Feb 24, 2016·Toxicon : Official Journal of the International Society on Toxinology·Robert Alvin Bernedo-Navarro, Tomomasa Yano
Dec 21, 2019·Immunological Investigations·Marzieh SanaeiMohammad Hossein Yazdi

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