Screening and Identification of Trichoderma Strains Isolated from Natural Habitats with Potential to Cellulose and Xylan Degrading Enzymes Production

Polish Journal of Microbiology
Roman MarecikAgnieszka Piotrowska-Cyplik

Abstract

A total of 123 Trichoderma strains were isolated from different habitats and tested for their ability to degrade cellulose and xylan by simple plate screening method. Among strains, more than 34 and 45% respectively, exhibited higher cellulolytic and xylanolytic activity, compared to the reference strain T. reesei QM 9414. For strains efficiently degrading cellulose, a highest enzyme activity was confirmed using filter paper test, and it resulted in a range from 1.01 to 7.15 FPU/ml. Based on morphological and molecular analysis, the isolates were identified as Trichoderma. The most frequently identified strains belonged to Trichoderma harzianum species. Among all strains, the most effective in degradation of cellulose and xylose was T. harzianum and T. virens, especially those isolated from forest wood, forest soil or garden and mushroom compost. The results of this work confirmed that numerous strains from the Trichoderma species have high cellulose and xylan degradation potential and could be useful for lignocellulose biomass conversion e.g. for biofuel production.

References

Jan 1, 1977·Journal of General Microbiology·L Hankin, S L Anagnostakis
Jun 13, 2002·Bioresource Technology·Ye Sun, Jiayang Cheng
May 6, 2003·Analytical Biochemistry·Henning JørgensenLisbeth Olsson
Nov 11, 2003·Bioresource Technology·Xia Liming, Shen Xueliang
Oct 20, 2004·Bioresource Technology·Zhiyou WenShulin Chen
Jun 10, 2005·Applied Microbiology and Biotechnology·M L T M PolizeliD S Amorim
Jun 14, 2005·Progress in Biophysics and Molecular Biology·Mats SandgrenColin Mitchinson
Aug 5, 2005·Annual Review of Phytopathology·Jae-Hyuk Yu, Nancy Keller
Sep 13, 2005·Fungal Genetics and Biology : FG & B·Irina S DruzhininaChristian P Kubicek
Jul 5, 2008·Bioresource Technology·A T W M Hendriks, G Zeeman
Dec 23, 2008·Biotechnology Advances·Carmen Sánchez
Mar 28, 2009·International Journal of Molecular Sciences·Mohammad J Taherzadeh, Keikhosro Karimi
Jun 9, 2009·Current Opinion in Biotechnology·David B Wilson
Apr 6, 2011·Journal of Applied Genetics·Lidia BłaszczykMarek Siwulski
Oct 14, 2011·Fungal Diversity·Walter M Jaklitsch
Feb 22, 2012·Waste Management·K ZiemińskiM Kowalska
May 18, 2012·Water, Air, and Soil Pollution·Paweł CyplikLukasz Chrzanowski
Jun 22, 2012·The ISME Journal·Thomas W CrowtherT Hefin Jones
Nov 30, 2012·Folia Microbiologica·Lidia BłaszczykDorota Frużyńska-Jóźwiak
Dec 3, 2013·Current Genomics·Antonella AmoreVincenza Faraco
Jul 8, 2015·Nature Communications·Tien-Chye TanChristina Divne
Jan 1, 2016·3 Biotech·Hillol ChakdarAlok Kumar Srivastava

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Citations

Oct 31, 2018·Bioengineering·Rajeev RavindranAmit K Jaiswal
Dec 29, 2020·Biotechnology Reports·Achmad Syafiuddin, Mohamad Ali Fulazzaky
Apr 14, 2021·Environmental Science and Pollution Research International·Sarmistha SarangiSayaji T Mehetre

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Methods Mentioned

BETA
chemical
biochemical treatment

Software Mentioned

BLASTn
Statistica

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