Screening and selection of Bifidobacterium strains isolated from human feces capable of utilizing resistant starch

Journal of the Science of Food and Agriculture
Hyun-Joong KimChang Joo Lee

Abstract

Resistant starch (RS) has been studied for its ability to serve as a substrate for the microbiota present in the human large intestine and for its beneficial physiological effects. The aim of this study was to screen and select novel strains of lactic acid bacteria (LAB) in the genus Bifidobacterium isolated from human fecal samples for further application as probiotics relying on their utilization of RS3, a prebiotic. LAB were isolated from human fecal samples, based on their ability to utilize RS3 as a carbon source. Consequently, two LAB were identified as Bifidobacterium adolescentis based on morphological, physiological and biochemical properties, and molecular biological analysis. The RS3-utilizing ability of these isolates was shown by the rapid decrease in pH of RS3-MRS media and by the pinhole traces on the surface of RS3 particles. Isolated B. adolescentis JSC2 was shown to be negative for β-glucuronidase, suggesting that it would be safe for human use, and was found to be tolerant towards the acidic, bile-salt environment. This synbiotics approach of B. adolescentis JCS2, an RS-utilizing probiotics, coupled with RS utilization, is expected to enhance RS utilization in the food industry and be beneficial for the promo...Continue Reading

References

Jan 1, 1991·Journal of Bacteriology·W G WeisburgD J Lane
Jun 1, 1990·The Journal of Applied Bacteriology·P ChevalierP Ward
Jun 1, 1995·The Journal of Nutrition·G R Gibson, M B Roberfroid
Apr 1, 1996·Applied and Environmental Microbiology·R F WangC E Cerniglia
Jun 16, 1999·European Journal of Clinical Nutrition·H HoveP B Mortensen
Jul 13, 2000·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Z ZhangW Miller
Feb 13, 2001·Journal of Applied Microbiology·R G CrittendenM J Playne
Jul 27, 2001·Applied and Environmental Microbiology·R CrittendenP Myllärinen
May 16, 2006·Journal of Applied Microbiology·S ParvezH-Y Kim
Mar 25, 2009·Journal of Microbiology and Biotechnology·Sung-Mee Lim, Dong-Soon Im
Sep 1, 2009·The Journal of Nutritional Biochemistry·Cindy D Davis, John A Milner
Apr 27, 2010·Current Opinion in Biotechnology·I JankovicA Mercenier
Sep 19, 2012·Critical Reviews in Biotechnology·Parmjit S PanesarReeba Panesar
Jan 1, 2006·Comprehensive Reviews in Food Science and Food Safety·M G SajilataPushpa R Kulkarni

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Citations

Dec 18, 2020·Comprehensive Reviews in Food Science and Food Safety·Mustapha MbyeMutamed M Ayyash
Nov 26, 2019·Current Opinion in Biotechnology·Peter DeMartino, Darrell W Cockburn

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