PMID: 14632992Nov 25, 2003

Screening and selection of exopolysaccharide-producing strains of Lactobacillus delbrueckii subsp. bulgaricus

Journal of Applied Microbiology
A D WelmanR H Archer

Abstract

The selection of exopolysaccharide (EPS)-producing strains of Lactobacillus delbrueckii subsp. bulgaricus. Improved EPS-overproducing strains of L. delbrueckii subsp. bulgaricus were derived by chemical mutagenesis and selection. Initial screening of the chemically induced mutant pool relied primarily on the selection of strains with raised levels of lactic acid and reduced biomass formation. Supporting selection criteria used were ropiness and colonial mucoidy. Final screening of candidate strains undertaken in a semi-defined medium in batch culture, resulted in the selection of a mutant with a 35% improvement in specific EPS yield relative to the parent strain. Initial selection of mutants of L. delbrueckii subsp. bulgaricus on the basis of enhanced formation of lactate and reduced biomass formation, coupled with a ropy or mucoid phenotype, proved to be a satisfactory means of isolating strains with the potential for a higher level of specific EPS production than the parent strain. The assay protocol allowed for the selection of an EPS-overproducing strain of L. delbrueckii subsp. bulgaricus. Such strains are useful for the purposes of metabolic studies related to EPS-production.

References

Aug 25, 2007·International Journal of Food Microbiology·Roel Van der MeulenLuc De Vuyst
Jun 9, 2016·International Journal of Molecular Sciences·Huu-Thanh NguyenFrank Delvigne
Apr 27, 2019·Food Science & Nutrition·Khubaib AliMuhammad Naveed Sheas
Dec 5, 2018·Food Science & Nutrition·Oniovosa Leonard Adamu-GovernorSylvia Veronica Ajagugha Uzochukwu

Citations

Oct 1, 1996·Antonie van Leeuwenhoek·W M de Vos
May 26, 1998·International Journal of Food Microbiology·S A Kimmel, R F Roberts
Jun 29, 2002·Nature Biotechnology·Ranjan PatnaikStephen del Cardayré
Apr 1, 1961·The Journal of Applied Bacteriology·B P EDDY
Feb 1, 1987·Applied and Environmental Microbiology·P P Renault, H Heslot

Related Concepts

Lactate
Radionuclide Metabolic Studies
Metabolic Abnormality Assessment
Lactic Acid Measurement
Polysaccharides, Bacterial
Chemically Induced
Lactates
Bacteriological Techniques
Food Microbiology
Mutant

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