Screening Cherry Germ Plasm for Resistance to Leaf Spot

Plant Disease
Phillip S WhartonAlan L Jones

Abstract

A detached leaf disk assay was developed for screening sour cherry (Prunus cerasus) cultivars for resistance to cherry leaf spot (Blumeriella jaapii). This assay was used to characterize the events occurring in cv. Montmorency (susceptible) and cv. GiSelA 6 [GI 148-1] (resistant) host-pathogen interactions, and to develop a disease scoring scheme to categorize disease severity in sour cherry trees. Forty-three seedlings grown from seeds collected in Russia were screened for resistance using the scoring scheme. Cross infection studies were also carried out with leaf spot isolates from four other Prunus species. These studies were made possible by the development of a new method of culturing B. jaapii isolates, using cherry fruit agar. This method enabled large amounts of inoculum to be produced within 2 weeks rather than a couple of months as required previously. In 'Montmorency', disease symptoms were first observed 4 days postinoculation (dpi), with the appearance of small white spots on the undersides of inoculated leaves. These spots gradually grew, producing erumpent spore masses in acervuli approximately 7 dpi. In 'GI 148-1', disease symptoms were also first observed 4 dpi, with the appearance of small transparent lesions....Continue Reading

References

Dec 1, 1979·The Australian and New Zealand Journal of Psychiatry·G Parker, L B Brown
Oct 24, 2008·Phytopathology·P S WhartonR J O'Connell

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