Screening, discovery, and characterization of angiotensin-I converting enzyme inhibitory peptides derived from proteolytic hydrolysate of bitter melon seed proteins

Journal of Proteomics
Anugerah Dany PriyantoJue-Liang Hsu

Abstract

In this study, new angiotensin-I converting enzyme (ACE) inhibitory peptides were comprehensively identified from a thermolysin digest of bitter melon (Momordica charantia) seed proteins. The hydrolysate was fractionated by reversed-phase high performance liquid chromatography (RP-HPLC), and the inhibitory activities of the resulting fractions were evaluated using ACE inhibitory assay. Two novel ACE inhibitory peptides (VY-7 and VG-8) were identified using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and database-assisted peptide sequencing. VY-7 and VG-8 were derived from momordin A and MAP30, respectively, and their IC50 values were as low as 8.64±0.60 and 13.30±0.62 μM, respectively. Lineweaver-Burk plots further indicated that VY-7, which showed the best IC50 value, acts as a competitive inhibitor. Notably, the content of VY-7 in crude thermolysin digest was determined to be as high as 14.89±0.88 μg/mg using LC-MS/MS quantification. In the spontaneously hypertensive rat (SHR) model, oral administration of VY-7 at 2mg/kg body weight significantly decreased the systolic blood pressure. The interaction between VY-7 and ACE was examined using molecular docking calculations and the results suggested that certain res...Continue Reading

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Citations

Mar 24, 2017·Nutrients·Ceren Daskaya-DikmenBeraat Ozcelik
Jun 24, 2017·Journal of Complementary & Integrative Medicine·Mohammad Yaseen Khan, Vimal Kumar
Jul 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dwi Yuli PujiastutiJue-Liang Hsu
Nov 29, 2017·BioMed Research International·César Ozuna, Ma Fabiola León-Galván
Jan 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Julio Caballero
Nov 21, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cesarea Hulda JoelJue-Liang Hsu
Dec 20, 2020·Food Chemistry·Arshdeep KaurSawinder Kaur
Feb 16, 2021·Molecular and Cellular Biochemistry·Bhoomika PatelMahaveer Dhobi
May 24, 2021·Peptides·Naohisa Shobako
Oct 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Samuchaya NgamsukJue-Liang Hsu
Jul 3, 2019·Journal of Agricultural and Food Chemistry·Ya-Hui ShihJue-Liang Hsu
Sep 12, 2020·Journal of Agricultural and Food Chemistry·Yu-Tang WangYing-Hua Zhang
Nov 4, 2021·Phytotherapy Research : PTR·Elyas Nattagh-EshtivaniAbed Ghavami

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